Sequences from confirmation amplicon


Confirmation sequences for line 194A01 and hit At1g75440 are shown.
Line ID 194A01
Locus At1g75440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28314893
Primers used for PCR AB03 - 8474, show primer details
   
Sequence Name 25-K020436-022-194-A01C-8409
GenBank Accession LN497247 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K020436-022-194-A01C-8409
TGTAGATTTCCCGGACATGAAGCCATTTACATATCGGTCATTATCGGTTGGTCGTTGCTG
CAACAAAACAAGCCAAGCGAGACGAAGGTTCAGCTTCAGCAACAAAGAGATCAAAGTTTA
TATATTAATGGTGAGTGTAAGAGCTGTAAGTACCTTTTCAGTCGAGCTTGAGAGCATGGA
GAGGATGCTAATGCAGATAGAACTCACCGTCATGGCTGGAGACCACGAATCATACAAAAT
ATCTGCAACGATGATACACAACATCAGATACTTCTTCCAGGAGGCCTATGTTAAATAATA
AGCTACTGAAATTTGAGTGTACCCTTGGATCTAAAAGCAAAATCTCAAGCAATATGAGTT
TCGAAGAGGGTCTTATCTTCGGGTAAGACTTCAAATGAGTTTAGGTATACAAACGACTTA
TGTTACCTCTCACATTGTGATTATTAATATATTAGAGATCATACAATATAGGAGTTGTGT
GTTTATCATTCGGACCACATAGTATGACCAGCAAGTAAAGAAAGTGATAGATAATAACCA
ACAAAAGAGTAAGAAGGTATCAGAAAACAGAGGACACAGTGACATACCTAGACAAATATG
CCCATTGCTGTAAATGTGAGGATGCAGAGGAGCTGGATGAAGAAAAATCACCTGTACAAT
ATTTAAAAAAAAAA
   
Sequence Name 26-K020436-022-194-A01C-AB03
GenBank Accession LN497723 [GenBank]
Sequencing primer gene specific primer, AB03: 5'-TGATCTTTACCGAGTTTGGTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K020436-022-194-A01C-AB03
GCCTTGTCATGGAATGTTTTGATCTGTCTCGAGTTGACTTGAGTTCTCTGTTCTTTACTT
CTTGTTTGCGTTTGTAATGTTTTTTTTTTTTTAAAAATTGAACAGGGGATTTTTCTTCAT
CCAGCTCCTCTGCATCCTCACATTTACACCAAGGGGCAAATTTGTCAAGGAATGTCCCTG
GGCCCTCTGTTTTCTGAAACCTTCTAACTCTTTTGTGGGTAATAATCAATCCCTTTCTTT
ACTTGCTGGCCAAACAATGGGGCCCAAATGAAAAACCCCCAACTCCAAAATTGTATGATC
TCTAAAAAATAAAAAATCCCAATGGGAAAGGAAACAAAAGCCGTTTGTATACCTAAACTC
ATTTGAAGTCTTACCCAAAAAAAAAACCCTTTTCAAAACTCAAATTGCTTGAAATTTTGC
TTTTAAATCCAGGGGTACCCTCAAATTTCAGAACCTTATTATTTAACATAGGCCTCCTGG
AAAAAGAATCTGATGTTGGGAATCACCGTTGCAAAAATTTTGAATGATTCGGGGCCTCCA
CCCATGACGGGGAGTTCTATCTGCATTAGCATCCTCTCCATGTTCTCAAGCTCGACTGAA
AA

Last Updated on 06.12.2018 16:11