Sequences from confirmation amplicon


Confirmation sequences for line 194B10 and hit At4g28700 are shown.
Line ID 194B10
Locus At4g28700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14161543
Primers used for PCR CW01 - 8474, show primer details
   
Sequence Name 45-K019824-022-194-B10C-CW01
GenBank Accession LN505376 [GenBank]
Sequencing primer gene specific primer, CW01: 5'-GACCCGTCAAGAAAGAAGAATAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K019824-022-194-B10C-CW01
GCAACGAACTGAGTCCTCTCCGCTATCGAACCACTCGTGATTCCAGCGGCGGCGATAGCG
AAAGTCCATTGATATAGAAAATAAGGATAATCAAACGTAGGTTGAGGAAAATCATACATT
CCAAAGAAATGTTTTCCGATGAATCCATTAGAAGGAGATCCAAAAGCAAAAGCGAAACCG
AAGAGATAATAGAAGAGACCTCCGGCTGCAGCGTCGATGACGTTCGTGAGCATAATGTTC
ATCGTGTTTTTTGCACGTACGGATCCGGCACAGAGCATGGCGAAACCGAGCTGCATCGCG
AAAACGAGGTAAGCAGAGAAGAGAAGGTAAGTGTTGTCGATTGCGTAAGCCGCATCAGTG
AATTTCCCGGCGACTGTGTCGAATCTCCCGCAGATGTATTCAGCGGCGGCTGCTGCTGCG
GTGGCGTTGGCTCCACCTGATAGTAATGGAATCAGATCAGAGGCAGAGCAAGAGAGAGCC
GACGCCATTGTTGCAAAGATTAAGAGAGATTTTGTGAGAGATGAAGAAGAGTCAGAGAGT
AGAGTTTATAGAGGAGGAAGGTAGAGAGATTCACGACATTCTGTTGAATCATTATTAGAG
AATTTGAATCTTTCAGATTCTATTCTGTAACCCTAAATTACTATAGGATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
   
Sequence Name 46-K019824-022-194-B10C-8409
GenBank Accession LN505724 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K019824-022-194-B10C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTATAGTAATTTAGGGTTA
CAGAATAGAATCTGAAAGATTCAAATTCTCTAATAATGATTCAACAGAATGTCGTGAATC
TCTCTACCTTCCTCCTCTATAAACTCTACTCTCTGACTCTTCTTCATCTCTCACAAAATC
TCTCTTAATCTTTGCAACAATGGCGTCGGCTCTCTCTTGCTCTGCCTCTGATCTGATTCC
ATTACTATCAGGTGGAGCCAACGCCACCGCAGCAGCAGCCGCCGCTGAATACATCTGCGG
GAGATTCGACACAGTCGCCGGGAAATTCACTGATGCGGCTTACGCAATCGACAACACTTA
CCTTCTCTTCTCTGCTTACCTCGTTTTCGCGATGCAGCTCGGTTTCGCCATGCTCTGTGC
CGGATCCGTACGTGCAAAAAACACGATGAACATTATGCTCACGAACGTCATCGACGCTGC
AGCCGGAGGTCTCTTCTATTATCTCTTCGGTTTCGCTTTTGCTTTTGGATCTCCTTCTAA
TGGATTCATCGGAAAACATTTCTTTGGAATGTATGATTTTCCTCAACCTACGTTTGATTA
TCCTTATTTTCTATATCAATGGACTTTCGCTATCGCCGCCGCTGGAATCACGAGTGGTTC
GATAGCGGAGAGGACTCAGTTCGTTGCGTATTTGATCTATTCTT

Last Updated on 06.12.2018 16:11