Sequences from confirmation amplicon


Confirmation sequences for line 194D09 and hit At2g32960 are shown.
Line ID 194D09
Locus At2g32960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13990579
Primers used for PCR 04ea - 8474, show primer details
   
Sequence Name 26-K110359-0022-194-D09C-04ea
GenBank Accession LN497977 [GenBank]
Sequencing primer gene specific primer, 04ea: 5'-ACAGTACTCTAGGGAACAGCCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K110359-0022-194-D09C-04ea
GATGAATATAGCCGACACATCTCATCATAATTTGGATATATTTTTTGCTGGCTTGTGCGT
TACTAAAGACTTACCAAGGCACTGATAGTGTGATCCTCATTTTTGCAGGAGCCATTTGTA
GATATCCTAGATCAGAAAATCCGTGAAGCACTCAAAGTCCTTCTCGGTAAAAATCATAGA
AGCCCTAAAACAAAGTTAAGAGAAAATCATATGTTTTGGTTATATATACAAGTCTTCTAA
TCCTGTCTTCTTTGTTAATCCTCAGATGAGAAGAACCATCCACTTTTGATTCATTGCAAA
CGAGGCAAGGTATATGTAAAATAGTAAATATCAAAAGCTATAAATCTCCTTAAATGATTT
TCATTTTCTTTGTATTGACTCAACACAATGGTTTATATATGATCATTGCAGCACAGAACC
GGGTGTCTTGTTGGGTGCATGAGGAAACTCCAGAAATGGTGCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 10-K110358-0022-194-D09C-8409
GenBank Accession LN490707 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K110358-0022-194-D09C-8409
TTTCTGGAGTTTCCTCATGCACCCAACAAGACACCCNGGTTCTGTGCTGCATTGATCATA
TATAAACCATTGTGTTGAGTCAATACAAAGAAAATGAAAATCATTTAAGGAGATTTATAG
CTTTTGATATTTACTATTTTACATATACCTTGCCTCGTTTGCAATGAATCAAAAGTGGAT
GGTTCTTCTCATCTGAGGATTAACAAAGAAGACAGGATTAGAAGACTTGTATATATAACC
AAAACATATGATTTTCTCTTAACTTTGTTTTAGGGCTTCTATGATTTTTACCGAGAAGGA
CTTTGAGTGCTTCACGGATTTTCTGATCTAGGATATCTACAAATGGCTCCTGCAAAAATG
AGGATCACACTATCAGTGCCTTGGTAAGTCTTTAGTAACGCACAAGCCAGCAAAAAATAT
ATCCAAATTATGATGAGATGTGTCGGCTATATTCATTCTTAGAGACCGAAATTGATCAGA
AAACTAGATTGGTTTTGGCTGTTCCCTAGAGTACT

Last Updated on 06.12.2018 16:11