Sequences from confirmation amplicon


Confirmation sequences for line 194E01 and hit At1g66070 are shown.
Line ID 194E01
Locus At1g66070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24596514
Primers used for PCR 12uv - 8474, show primer details
   
Sequence Name 71-K117671-0022-194-E01C-12uv
GenBank Accession LN512623 [GenBank]
Sequencing primer gene specific primer, 12uv: 5'-TTCCAACTATATATTTCCGCAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K117671-0022-194-E01C-12uv
AATTAGAAAATGTGTTTCTTTTGAGTGATAGATAAATGATAAAATAGACGCTGGACAAAT
CCAATTTGCATTTGCTCAAAAAGCTCTTTTAATCTACATGAAGTCGAAGTCATCCATGGC
ATCATAAGGACCAGCCACTAGATCGTCATTGGCCTTATCTACAATAAGTTGTTTCTTCTT
TCCACCTGATCAAGCAATGCCAATGGTCATGAAAAACGGATTCAGAATAACACATTCATA
AAACTATAAACCAAAGATGAAGTGATTGTGAGTAACACATTCAGAATAATACCTTTCTTT
TTTCCCGCAGCAGCTTCTTTCTCTGCCTTTAGTTTTTCATTTGCTATCGTTGTAATGGAC
GAAGCAACATCTTTAACATCAGCTGCTTTCATGTTGGTCAATGAAAGTCTACATGGATAT
ATTTTGAGATTCATGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTAT
TAT
   
Sequence Name 23-K117670-0022-194-E01C-8409
GenBank Accession LN496731 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K117670-0022-194-E01C-8409
TCTCAATATTCCATGTAGACTTTCATTGACCAACATGAAAGCAGCTGATGTTAAAGATGT
TGCTTCGTCCATTACAACGATAGCAAATGAAAAACTAAAGGCAGAGAAAGAAGCTGCTGC
GGGAAAAAAGAAAGGTATTATTCTGAATGTGTTACTCACAATCACTTCATCTTTGGTTTA
TAGTTTTATGAATGTGTTATTCTGAATCCGTTTTTCATGACCATTGGCATTGCTTGATCA
GGTGGAAAGAAGAAACAACTTATTGTAGATAAGGCCAATGACGATCTAGTGGCTGGTCCT
TATGATGCCATGGATGACTTCGACTTCATGTAGATTAAAAGAGCTTTTTGAGCAAATGCA
AATTGGATTTGTCCAGCGTCTATTTTATCATTTATCTATCACTCAAAAGAAACACATTTT
TCTTAATTTTGGTTTGAATAGAGCAAGAAGAGCCAAATTTCTGGTTGCGGAAATATATAG
TTGGA

Last Updated on 06.12.2018 16:11