Sequences from confirmation amplicon


Confirmation sequences for line 195B04 and hit At1g80210 are shown.
Line ID 195B04
Locus At1g80210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:30164788
Primers used for PCR KR03 - 8474, show primer details
   
Sequence Name 56-K030600-022-195-B04C-KR03
GenBank Accession LN509001 [GenBank]
Sequencing primer gene specific primer, KR03: 5'-TCCTGAGTTTCAAATCTTGTTGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K030600-022-195-B04C-KR03
TTGGAACTGACTTATACTCAAAGGAACGGGGGAAGTGCCACAGCTATGATCTGGGGAGCA
TCGCCTCAGTCACGGTCTGATCGGCAGAAGGATAGAGTTGAAACCAATCCTGAACAACTG
GCTGCTGCTTCTGCTCAGGCTGATATATCCTTTTTATAAACTTGCTTTTTTCAGATTGAT
GGTCTATGTGTATTTTAGTTATGGTTTGGTTAAAACAAGAGTGTTTTGTAGTCTTCCTTC
ACTAATTTCGACACAGAATGACGATATCAACGGGCAGAACTACACGGGTCATTGGATGGT
ACCACTCCCATCCTCATATTACCGTTCTTCCTTCCCATGTCGGTATGTATCATCATCATT
GTAACTTCTCGATGTTTTTATCTTGTTCCTCATATTTATGTCCTGTTTCTGCCTGCAGAT
GTTCGAACACAAGCTATGTATCAGCTTTTAGATTCTGGTTTTATTGGCCTCATCTTTTCC
TGTTTTAGTGAAGATGCTAACAAGGTTAGTTAGTATTTGCTATGAGTGGATTAGCTACTT
GAAACTTTTCAAAAATACTTCTCTTCAGCAATTGTAAATGGCTTCATGTC
   
Sequence Name 17-K030600-022-195-B04C-8409
GenBank Accession LN493732 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K030600-022-195-B04C-8409
GCATTCTTTCACTAAAAACATGGAAAATGAATGATGGCCAAATAAAAACCAGAAATCTAA
AAGCTGATACATAGCTTGTGTTCGAAACATCTGCAGGCAGAAACAGGACATAAATATGAG
GAAACAAGATAAAAACATCGAGAAGTTACAATGATGATGATACATACCGACATGGGAAGG
AAGAAACGGTAATATGAGGATGGGAGTGGTACCATCCAATGACCCGTGTAGTTCTGCCCG
TTGATATCGTCATTCTGTGTCGAAATTAGTGAAGGAAGACTACAAAACACTCTTGTTTTA
ACCAAACCATAACTAAAATACACATAGACCATCAATCTGAAAAAAGCAAGTTTATAAAAA
GGATATATCAGCCTGAGCAGAAGCAGCAGCCAGTTGTTCAGGATTGGTTTCACTCTATCT
TCTGCGATAGACGTGACTGAGCGATGCTCCCAATCATACTGTGCACT

Last Updated on 06.12.2018 16:11