Sequences from confirmation amplicon


Confirmation sequences for line 195C03 and hit At1g65870 are shown.
Line ID 195C03
Locus At1g65870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24504150
Primers used for PCR 37vs - 8474, show primer details
   
Sequence Name 37-K117953-0022-195-C03C-37vs
GenBank Accession LN502772 [GenBank]
Sequencing primer gene specific primer, 37vs: 5'-GCCACCATTACAGAATCAAAATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K117953-0022-195-C03C-37vs
ATATCCGGACATAAACCGGATAAGCTAACCCACTTACACTTCTACTTCCACGACATTGTC
TCCGGAGACAAACCAACCTCCGTCCAAGTCGCTAACGGTCCAACCACAAACTCTTCTGCC
ACCGGTTTCGGTTTGGTTGCAGTCGTTGACGACAAATTGACGGTCGGGCCTGAAATTACC
TCAGAGGAAGTTGGGAGAGCCCAAGGAATGTATGCATCGGCTGACCAGAACAAGCTTGGT
CTGCTTATGGCTTTTAACTTGGTTTTCACGAAGGGTAAATTTTCCGACAGCACCGTGGCG
ATGTATGGCCGGAATCCTGTATTGTCGAAGGTGAGGGAAATGCCGATCATTGGAGGTACG
GGAGCTTTCAGGTTCGGAAGAGGATATGCATTGGCCAAGACTTTGGTGTTCAATATTACC
AGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATATCCGCACGGTTATTAT
   
Sequence Name 13-K117952-0022-195-C03C-8409
GenBank Accession LN492238 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K117952-0022-195-C03C-8409
GCCATTTAATTGAATATATCCTGGTAATATTGAACACAAAGTCTTGGCCAATGCATATCC
TCTTCCGAACCTGAAAGCTCCCGTACCTCCAATGATCGGCATTTCCCTCACCTTCGACAA
TACAGGATTCCGGCCATACATCGCCACGGTGCTGTCGGAAAATTTACCCTTCGTGAAAAC
CAAGTTAAAAGCCATAAGCAGACCAAGCTTGTTCTGGTCAGCCGATGCATACATTCCTTG
GGCTCTCCCAACTTCCTCTGAGGTAATTTCAGGCCCGACCGTCAATTTGTCGTCAACGAC
TGCAACCAAACCGAAACCGGTGGCAGAAGAGTTTGTGGTTGGACCGTTAGCGACTTGGAC
GGAGGTTGGTTTGTCTCCGGAGACAATGTCGTGGAAGTAGAAGTGTAAGTGGGTTAGCTT
ATCCGGTTTATGTCCTGGATATGGTGCCTTCACTGTTGTTGAGAAAGATTTTGATTCTGT
AATGGTGGC

Last Updated on 06.12.2018 16:11