Sequences from confirmation amplicon


Confirmation sequences for line 195D11 and hit At5g10490 are shown.
Line ID 195D11
Locus At5g10490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3303225
Primers used for PCR PG18 - 8474, show primer details
   
Sequence Name 50-K101746-0022-195-D11C-8409
GenBank Accession LN507114 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K101746-0022-195-D11C-8409
GCCATTTACAATTGAATATATCCTGGAAATTACGAGAAACATAAATGCACCCCCTCAGAT
TTCCCCACCAAAAAAATTACAACTTCCCTAAGGGGAGCCATGGTAATGACAAGGCCTGCA
TACATCAATGTTTGGCTCAAGTCCACAATGTTTATGAGTAGAGTTAATTCATTCATTTTG
AGTTAGATGCCCAAATATTACTATGCAATCATTCAGATCCATGAAGATCAACTAAATAAA
AATAATGATAGTTTCCAATTTTACAAGGTGGTCATTCTGCATGTGATGATAGGAAACAAG
GAGTCTTACTTTGTTAGCACCACGGTGACAGCTTTAACAGCAGGTTCAATTGCCTTGCCG
CTTGCAGAAAAGGAATGACACCTAANAGCGGTGGTTCTGCATGGAACTGAACAAATA
   
Sequence Name 50-K101752-0022-195-D11C-PG18
GenBank Accession LN507115 [GenBank]
Sequencing primer gene specific primer, PG18: 5'-CTATTTGTTCAGTTCCATGCAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K101752-0022-195-D11C-PG18
TCTTTTCGCAGCGGCAGGCAATTGAACCTGCTGTTAAAGCTGTCACCGTGCTGCTAACAA
AGTAAGACTCCTTGTTTCCTATCATCACATGCAGAATGACCACCTTGTAAAATTGGAAAC
TATCATTATTTTTATTTAGTTGATCTTCATGGATCTGAATGATTGCATAGTAATATTTGG
GCATCTAACTCAAAATGAATGAATTAACTCTACTCATAAACATTGTGGACTTGAGCCAAA
CATTGATGTATGCAGGCCTTGTCATTACCATGGCTCCCCTTAGGGAAGTTGTAATTTTTT
TGGTGGGGAAATCTGAGGGGGTGCATTTATGTTTCTCGTAATTTCCAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
Primers used for PCR PG19 - 8474, show primer details
   
Sequence Name 51-K101746-0022-195-D11C-OriL
GenBank Accession LN507451 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K101746-0022-195-D11C-OriL
ATTTACAATTGAATATATCCTGTCCGTTTTTTGCAGGTCACACGGGTTGATGCAACAATT
CCCTTTTGTATATAAATTGGTTCCAGCTGTTGCTCTTCTTGTTTTCTCCCTGTGGGGTCT
AGTGCCTTTTGCTCGCCAAGGAAGAAACATACTACTCAATGTAGGTGCAGCCATTTCAAG
TTTATATGCACTTTTTTTTTGTTTACTGCTTTCATTTCTGTGTTGTCTCAGTTCAAATCA
ATGTGGTATCTTTTCTATATGAGATTCTGAATCTTTACCTTTGAATCATTGTAGAAAAAT
GATAACGGTTGGAAGAAGAGTGGTACGTACCATGTTATGACTTCATATGTTCAACCTCTG
TTACTATGGTTAGGAGCTCTATTCATCTGCAGGTATATCTATCAAGTAGTTCCTCGGGAC
TTTCTGAGATTTAATTGTTGAGATATTTTGGTGGTATCCTCATCTGAAGTGCTTGGTGAA
AATAATTG
   
Sequence Name 51-K101752-0022-195-D11C-PG19
GenBank Accession LN507452 [GenBank]
Sequencing primer gene specific primer, PG19: 5'-CAATTATCTCAACCAAGCACTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K101752-0022-195-D11C-PG19
TCTCAGAAAGTCCCGAGGAACTACTTGATAGATATACCTGCAGATGAATAGAGCTCCTAA
CCATAGTAACAGAGGTTGAACATATGAAGTCATAACATGGTACGTACCACTCTTCTTCCA
ACCGTTATCATTCTTCTACAATGATTCAAAGGTAAAGATTCAGAATCTCATATAGAAAAG
ATACCACATTGATTTGAACTGAGACAACACAGAAATGAAAGCAGTAAACAAAAAAAAAGT
GCATATAAACTTGAAATGGCTGCACCTACATTGAGTAGTATGTTTCTTCCTTGGCGAGCA
AAAGGCACTAGACCCCACAGGGAGAAAACAAGAAGAGCAACAGCTGGAACCAATTTATAT
ACAAAAGGGAATTGTTGCATCAACCCGTGTGACCTGCAAAAAACGGACAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTTCCG
CA

Last Updated on 06.12.2018 16:11