Sequences from confirmation amplicon


Confirmation sequences for line 196F07 and hit At1g69640 are shown.
Line ID 196F07
Locus At1g69640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26195625
Primers used for PCR NH02 - 8474, show primer details
   
Sequence Name 42-K036526-022-196-F07C-8409
GenBank Accession LN504381 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K036526-022-196-F07C-8409
GAGCCATTTACAATTGAATATATCCCTGTATATTTCCCTAAAACAATCCCCAATCATCGT
TTCAATAATCAAATTTCCCCAATTGAAGAATAAAGATCAAAACTTTGGAAGTTTTCCTGT
GAAAATAATCTATCCTTTTTGCATCATTTGCGTTTTGGTTGATGAAGAGGATCTGAAGAA
GCTGAGATTTAGGAATGATGATGGGTTTTGCTGTATCGGATGAATTGTTGGGTACTGTTG
CTCCGATTGTGGTGTACTGGTTGTATTCTGGGATTTATGTAGCGCTCTCCTCTTTAGAGA
GTTATAGATTGCATTCAAAGGTTGAAGAAGAAGAGAAGAATCTTGTATCGAAATCTTCTG
TTGTGAAAGGTGTTCTTGTTCAACAGGTTGTTCAAGCTGTTGTCGCTATCCTTTTGTTCA
CGGTACCGTTTTTCTCTTTTCCCATTTTTGAGTTGTTTTGCTTTTGTGAAATTGAAGTGA
AAAGTTAGGTAAAACATATTGGGGAATTTTGATATCTGACGCTGACTTTTTATGTGTGGA
ATTGATGGGTTAAG
   
Sequence Name 66-K036526-022-196-F07C-NH02
GenBank Accession LN511543 [GenBank]
Sequencing primer gene specific primer, NH02: 5'-CTTAACCATCCAATTCCACACATA-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K036526-022-196-F07C-NH02
TCAAATTCCCCAATATGTTTTACCTAACTTTTCACTTCAATTTCACAAAAGCAAAACAAC
TCAAAAATGGGAAAAGAGAAAAACGGTACCGTGAACAAAAGGATAGCGACAACAGCTTGA
ACAACCTGTTGAACAAGAACACCTTTCACAACAGAAGATTTCGATACAAGATTCTTCTCT
TCTTCTTCAACCTTTGAATGCAATCTATAACTCTCTAAAGAGGAGAGCGCTACATAAATC
CCAGAATACAACCAGTACACCACAATCGGAGCAACAGTACCCAACAATTCATCCGATACA
GCAAAACCCATCATCATTCCTAAATCTCAGCTTCTTCAGATCCTCTTCATCAACCAAAAC
GCAAATGATGCAAAAAGGATAGATTATTTTCACAGGAAAACTTCCAAAGTTTTGATCTTT
ATTCTTCAATTGGGGAAATTTGATTATTGAAACGATGATTGGGGATTGTTTTAGGGAAAT
ATACAGGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAA
ATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATC
TTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11