Sequences from confirmation amplicon


Confirmation sequences for line 198A01 and hit At5g55230 are shown.
Line ID 198A01
Locus At5g55230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22402792
Primers used for PCR CL67 - 8474, show primer details
   
Sequence Name 78-K033098-022-198-A01C-8409
GenBank Accession LN513630 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K033098-022-198-A01C-8409
ATCTTGCTACCATCTCCCCTAAGTATTAGGGCTTTACTCTCAATCTTGTGTAACGTCAAC
TTTTTTTTACTAAAGGAAATCTGGGATGAAGTTGGTGAGAGTGATGATGAACGAGACAAA
CTGCTTCTTCAGATAGAGCAAGAGTGTCTTGACGTTTACAAGAGAAAAGTCGAGCAGGCT
GCGAAATCCCGAGCTGAGCTTCTTCAAACCTTGTCAGATGCTAATGCTGAACTTTCCAGC
CTCACAATGTCTCTTGGAGACAAAAGCTTAGTTGGCATTGTAAGTTTTGTCTCAAGTTTA
GTAGTAGAAAATCTAAGTGAAGTGTTTTTTGTTTTTTAAGTGATGTAAGTCTTGATTCTT
CTGTTGTTGCAGCCGGATAAGTCTTCAGGAACGATTAAAGAACAACTTGCTGCAATAGCA
CCGGCTCTTGAACAACTGTGGCAACAGAAAGAGGAGAGAGTCCGAGAGTTCTCTGATGTA
CAATCACAGATTCAGAAGATATGTGGAGATATTGCTGGAGGTTTGAGCAATGAGGTTCCT
ATAGTCGATGAGTCTGATTTGTCACTGAAGAAATTAGACGATTTCCAGAGCCAACTCCAA
GAGCTCCAGAAAGAGAAGGTAAACATTGAGTTAGAAATCTCCGTATATGATATGTTGCTT
ATGTTTTCTTACCAC
   
Sequence Name 30-K033098-022-198-A01C-CL67
GenBank Accession LN499617 [GenBank]
Sequencing primer gene specific primer, CL67: 5'-AAAACAGAGCAGTTGGTAAGGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K033098-022-198-A01C-CL67
TTCTACTCATGTTTACCTTCTCTTTCTGGAGCTCTTGGAGTTGGCTCTGGAAATCGTCTA
ATTTCTTCAGTGACAAATCAGACTCATCGACTATAGGAACCTCATTGCTCAAACCTCCAG
CAATATCTCCACATATCTTCTGAATCTGTGATTGTACATCAGAGAACTCTCGGACTCTCT
CCTCTTTCTGTTGCCACAGTTGTTCAAGAGCCGGTGCTATTGCAGCAAGTTGTTCTTTAA
TCGTTCCTGAAGACTTATCCGGCTGCAACAACAGAAGAATCAAGACTTACATCACTTAAA
AAACAAAAAACACTTCACTTAGATTTTCTACTACTAAACTTGAGACAAAACTTACAATGC
CAACTAAGCTTTTGTCTCCAAGAGACATTGTGAGGCTGGAAAGTTCAGCATTAGCATCTG
ACAAGGTTTGAAGAAGCTCAGCTCGGGATTTCGCAGCCTGCTCGACTTTTCTCTTGTAAA
CGTCAAGACACTCTTGCTCTATCTGAAGAAGCAGTTTGTCTCGTTCATCATCACTCTCAC
CAACTTCATCCCAGATTTCCTTTAGTAAAAAAAAGTTGACGTTACACAAGATTGAGAGTA
AAGCCCTAATACTTAGGTGGAGATGGTAGCAAGATTTACCTGCAACTTCTCAAGTAAGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATT

Last Updated on 06.12.2018 16:11