Sequences from confirmation amplicon


Confirmation sequences for line 198F02 and hit At3g14690 are shown.
Line ID 198F02
Locus At3g14690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4937724
Primers used for PCR 06gd - 8474, show primer details
   
Sequence Name 68-K112062-0022-198-F02C-06gd
GenBank Accession LN512044 [GenBank]
Sequencing primer gene specific primer, 06gd: 5'-TAAACAATCTAACTTGATTCCGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K112062-0022-198-F02C-06gd
GAAGCCGGGTTGATGATTCTTCGGTGTTTTGCCATTTATCACCATCATAATTAGCGAGTC
CTTTAGCTATAATTGTGGCCAAAGGAAATGTATGCGGCTTTTGGAAATCATAAACTTTGT
TGAACACTTCCTTGATTTGCTCAGGATCCATTATTGTGATGGTTGGGATAGGTCCAAACC
ATGTAAAGTAAGTCCTTCCTGTTCCAACCACATAATAGCAATACAAAAGTTGAAACCTGT
TCTCTACATCAGATACTCTGCAACAGTTCTAAACTTCTAAACAGTGAAGAGAGATATATT
CATTTACATGGCAATCTATATGGCATTGACCATCATACTCATTGCCATTTACAATTGATC
CAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCACACGGTTATA
   
Sequence Name 52-K112045-0022-198-F02C-8409
GenBank Accession LN507859 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K112045-0022-198-F02C-8409
TGAAGTTTTCAACTGCTGCATAATATCTGATGTAGAGAACAGGTTTCAACTTTTGTATTG
CTATTATGTGGTTGGAACAGGAAGGACTTACTTTACATGGTTTGGACCTATCCCAACCAT
CACAATAATGGATCCTGAGCAAATCAAGGAAGTGTTCAACAAAGTTTATGATTTCCAAAA
GCCGCATACATTTCCTTTGGCCACAATTATAGCTAAAGGACTCGCTAATTATGATGGTGA
TAAATGGGCAAAACACCGAAGAATCATCAACCCGGCTTTTCATATAGAGAAGATCAAGGT
TTAGTAACCCGGAATCAAGTTAGATTGTTT

Last Updated on 06.12.2018 16:11