Sequences from confirmation amplicon


Confirmation sequences for line 200A03 and hit At5g02500 are shown.
Line ID 200A03
Locus At5g02500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:555809
Primers used for PCR DM18 - 8474, show primer details
   
Sequence Name 70-K101747-0022-200-A03C-8409
GenBank Accession LN512354 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K101747-0022-200-A03C-8409
CGGAATGAGCCTTTACAATTGAATATATCCTGACAGCTCTGTTCAGAGTGACATGAAATT
GTGGCCATTCAAGATTCAAGCCGGACCTGCCGATAAGCCAATGATCTACGTCGAATACAA
GGGTGAAGAGAAAGAGTTCGCAGCTGAGGAGATTTCTTCCATGGTTCTTATTAAGATGCG
TGAGATTGCTGAGGCTTACCTTGGTGTCACAATCAAGAACGCCGTTGTTACCGTTCCAGC
TTACTTCAACGACTCTCAGCGTCAGGCTACAAAGGATGCTGGTGTCATCGCTGGTTTGAA
CGTTATGCGAATCATCAACGAGCCTACAGCCGCCGCTATTGCCTACGGTCTTGACAAAAA
GGCTA
   
Sequence Name 70-K101753-0022-200-A03C-DM18
GenBank Accession LN512355 [GenBank]
Sequencing primer gene specific primer, DM18: 5'-GTAGCCTTTTTGTCAAGACCGTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K101753-0022-200-A03C-DM18
GTTCAAACCAGCGATGACACCAGCATCCTTTGTAGCCTGACGCTGAGAGTCGTTGAAGTA
AGCTGGAACGGTAACAACGGCGTTCTTGATTGTGACACCAAGGTAAGCCTCAGCAATCTC
ACGCATCTTAATAAGAACCATGGAAGAAATCTCCTCAGCTGCGAACTCTTTCTCTTCACC
CTTGTATTCGACGTAGATCATTGGCTTATCGGCAGGTCCGGCTTGAATCTTGAATGGCCA
CAATTTCATGTCACTCTGAACAGAGCTGTCAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
Primers used for PCR DM19 - 8474, show primer details
   
Sequence Name 71-K101747-0022-200-A03C-OriL
GenBank Accession LN512528 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K101747-0022-200-A03C-OriL
GCCTTGATCATTGAATAAAAAACGGATTTAGTAATAAATCAAGCAATAAAGATCTGAAGC
ATAATCAACAAAAATAGCAATAAATATCTAACAAACTTACAAATTCCAAAAACCAACAGA
TACTACTAGTCCAAGCAAGAAATTACTAAAATAAGCATAGTTGAATTAATATAAACAGGA
TCTAACCGATAAGATCTATAGATGAAACACTAAATCCAACATATACGATTGTTATCCAGA
TCTAACTAGATCTAACTACATCACGACTACAATGAACGAGATCTAGATTTCAAGTATAAG
AGAGCTAAGAAAACGAGACACGTACCGAAAACGGTGTTAACGGGGTTCATGGCGACCTGA
TTCTTAGCTGCGTCACCGATCAACCTCTCGGAGTCGGTGAAAGCAACGTAAGATGGCGTG
GTTCTGTTTCCTTGATCATTAGCAATGATCTCAACACGGTCGTGTTGCCATACTCCGACG
CAAGAGTAAGTGGTACCAAGATCGATACCGATNGCTGGTCCTTCTCCTTTACCCGACATT
TTTATCGGAG
   
Sequence Name 71-K101753-0022-200-A03C-DM19
GenBank Accession LN512529 [GenBank]
Sequencing primer gene specific primer, DM19: 5'-TTTCCAAATCTTCCGATAAAAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K101753-0022-200-A03C-DM19
GTATCGATCTTGGTACCACTTACTCTTGCGTCGGAGTATGGCAACACGACCGTGTTGAGA
TCATTGCTAATGATCAAGGAAACAGAACCACGCCATCTTACGTTGCTTTCACCGACTCCG
AGAGGTTGATCGGTGACGCAGCTAAGAATCAGGTCGCCATGAACCCCGTTAACACCGTTT
TCGGTACGTGTCTCGTTTTCTTAGCTCTCTTATACTTGAAATCTAGATCTCGTTCATCGT
AGTCGTGATGTAGTTAGATCTAGTTAGATCTGGATAACAATCGTATATGTTGGATTTAGT
GTTTCATCTATAGATCTTATCGGTTAGATCCTGTTTATATTAATTCAACTATGCTTATTT
TAGTAATTTCTTGCTTGGACTAGTAGTATCTGTTGGTTTTTGGAATTTGTAAGTTTGTTA
GATATTTATTGCTATTTTTGTTGATTATGCTTCAGATCTTTATTGCTTGATTTATTACTA
AATCCGTTTTTTATTCAATTGATCAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11