Sequences from confirmation amplicon


Confirmation sequences for line 200C12 and hit At4g01170 are shown.
Line ID 200C12
Locus At4g01170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:499746
Primers used for PCR 06fm - 8474, show primer details
   
Sequence Name 17-K111117-0022-200-C12C-06fm
GenBank Accession LN493904 [GenBank]
Sequencing primer gene specific primer, 06fm: 5'-CGGTGTACACTCTTCTACATCCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K111117-0022-200-C12C-06fm
ATCATGATCTGGTTCACCTTCACTTCGACTAGTGGATCCATTTCAGGTACCATTGTCGAT
TCTTTTAAAGTATCCATATCATCTTTCTGAAGATTTTCCATTGGCTCAGTTGTCGAATCC
CTAAGAGAATCTACAAGCCATGTGAGGTACCTCATGTAATCCTTATCCACAACCGCTTCA
CTACCCTCATTCTTCTTCCTTCTCCCTACAAAAACTTCTTTGCTCCTTGAACAAGTCTCA
TCTACATCATTAACCTCTGTAACTCTATTTACTCGCTCCACATGATCAATCTCCGCAACA
ACCCTCTTGGCTAGTTGAATCTCTTCTGCAGTTCTAACCAATGGAACTGTGTTGTCTCGT
TGACTGTCTTCTTGACTTCCAACCAATGGAACATCATCTCTTCTTAAAGTCTCAGTCTCA
GCCTTTGAATCAGAAAGTTTCCTAACTCGTTGTTGAATGTCTTCTTGTTGGGTTTCGAGT
AAAGGAACGTCTCTTGAAGCCTTTGCAGCATCTAACATCGCAATCCTCCGCTTTCTTCGT
TCAGCAACTCTGTTGTCTCGTTGAATGATGTCTTGGATTTTATCTCTTTCAGCCTTTAAC
CCAACAGTTTTCCTCTGGCCCTGTTGTGACCCAACAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCG
   
Sequence Name 01-K111116-0022-200-C12C-8409
GenBank Accession LN486629 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K111116-0022-200-C12C-8409
TCATTGAATTATCCTGTTGGGTCACAACAGGGCCAGAGGAAAACTGTTGGGTTAAAGGCT
GAAAGAGATAAAATCCAAGACATCATTCAACGAGACAACAGAGTTGCTGAACGAAGAAAG
CGGAGGATTGCGATGTTAGATGCTGCAAAGGCTTCAAGAGACGTTCCTTTACTCGAAACC
CAACAAGAAGACATTCAACAACGAGTTAGGAAACTTTCTGATTCAAAGGCTGAGACTGAG
ACTTTAAGAAGAGATGATGTTCCATTGGTTGGAAGTCAAGAAGACAGTCAACGAGACAAC
ACAGTTCCATTGGTTAGAACTGCAGAAGAGATTCAACTAGCCAAGAGGGTTGTTGCGGAG
ATTGATCATGTGGAGCGAGTAAATAGAGTTACAGAGGTTAATGATGTAGATGAGACTTGT
TCAAGGAGCAAAGAAGTTTTTGTAGGGAGAAGGAAGAAGAATGAGGGTAGTGAAGCGGTT
GTGGATAAGGATTACATGAGGTACCTCACATGGCTTGTAGATTCTCTTAGGGATTCGACA
ACTGAGCCAATGGAAAATCTTCAGAAAGATGATATGGATACTTTAAAAGAATCGACAATG
GTACCTGAAATGGATCCACTAGTCGAAGTGAAGGTTGAACCAGATCATGATTGGTCTGAT
TATATAATTGCGATGGGTGATTCGCCGTTTTTGGATGTAG

Last Updated on 06.12.2018 16:11