Sequences from confirmation amplicon


Confirmation sequences for line 201A05 and hit At3g58370 are shown.
Line ID 201A05
Locus At3g58370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21596501
Primers used for PCR BL91 - 8474, show primer details
   
Sequence Name 27-K105305-0022-201-A05C-BL91
GenBank Accession LN498382 [GenBank]
Sequencing primer gene specific primer, BL91: 5'-TTGGGTGATTAAGAATTTCTCCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K105305-0022-201-A05C-BL91
TCTGATCCATTTGTGATTGGTGGCTGCAATGGTACTTAAGAAACTAAAACTTTTTTTCTA
ACTGTATTAGTCCAATTTAGGTTTACGAGGACATCCACTTTTTTTTGCAACTGCTTTTTG
TTTGCAGGCATCTTCTGGCCTATCCAAAGGGAAATAAATTTAATAATAGTTTGTCTCTGT
ATCTGGTGGTTGATGATGCTAGAGCATTGCCTTGTGGATGGAGAAGATATGCACAATTCT
CCTTAACTATAATAAATCAACTTACGGACAAACTCTCCCAACGAGGAGATAATTCTTTAT
TTCTAAACTTTATATTATAAAGGATATTTTATGATTCTACAACTTGTCATGGACACTCAT
GTGGCTTTGTGCTGCTGCAAGATTCTTGTTATGCATTGTGCAATTTTATTGCTTGTTTGG
ATTAGAGTTTCTATATATTTCGTGAACAGAGAAACAAAATTGGTTTAATCAAAGAAATCT
TGGCTCGGGTTTTACATCTATGATCCCTCTTCCCAACCTTCATGCCAAAAATGCTGGTCA
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCG
   
Sequence Name 11-K105306-0022-201-A05C-8409
GenBank Accession LN491107 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K105306-0022-201-A05C-8409
ATTTACATTGAATATATTGACCAGCATTTTTGGCATGAAGGTTGGGAAGAGGGATCATAG
ATGTAAAACCCGAGCCAAGATTTCTTTGATTAAACCAATTTTGTTTCTCTGTTCACGAAA
TATATAGAAACTCTAATCCAAACAAGCAATAAAATTGCACAATGCATAACAAGAATCTTG
CAGCAGCACAAAGCCACATGAGTGTCCATGACAAGTTGTAGAATCATAAAATATCCTTTA
TAATATAAAGTTTAGAAATAAAGAATTATCTCCTCGTTGGGAGAGTTTGTCCGTAAGTTG
ATTTATTATAGTTAAGGAGAATTGTGCATATCTTCTCCATCCACAAGGCAATGCTCTAGC
ATCATCAACCACCAGATACAGAGACAAACTATTATTAAATTTATTTCCCTTTGGATAGGC
CAGAAGATGCCTGCAAACAAAAAGCAGTTGCAAAAAAAAGTGGATGTCCTCGTAAACCTA
AATTGGACTAATACAGTTAGAAAAAAAGTTTTAGTTTCTTAAGTACCATTTGCAGCCACC
AATCACAAATGGATCAGAATAAATTTTGTCATATTGCAAAGTGGAGAAATTCTTANTCAC
CCA

Last Updated on 06.12.2018 16:11