Sequences from confirmation amplicon


Confirmation sequences for line 201C06 and hit At4g30960 are shown.
Line ID 201C06
Locus At4g30960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15067743
Primers used for PCR JK02 - 8474, show primer details
   
Sequence Name 27-K020436-022-201-C06C-8409
GenBank Accession LN498186 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K020436-022-201-C06C-8409
TGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCAAGGAAGACTACGTGA
AGACGTAGCTCGTGTTTATTTCCAGCAATTGATCTCAGCCGTTGATTTCTGTCATAGCCG
TGGTGTTTATCACCGAGATCTGAAACCGGAGAATCTCTTGTTAGATGAAGAAGGTAATCT
CAAGGTGACTGATTTTGGTCTCTCTGCTTTCACTGAGCATTTGAAACAAGACGGACTTCT
TCATACAACTTGTGGAACTCCGGCTTATGTTGCGCCGGAGGTTATATTGAAGAAAGGATA
CGACGGAGCTAAGGCGGATCTGTGGTCTTGTGGTGTTATACTTTTTGTGCTTCTTGCTGG
TTATTTGCCGTTTCA
   
Sequence Name 28-K020436-022-201-C06C-JK02
GenBank Accession LN498655 [GenBank]
Sequencing primer gene specific primer, JK02: 5'-GATTATCATCTTGAAACGGCAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K020436-022-201-C06C-JK02
AGCAAGAAGCACAAAAAGTATAACACCACAAGACCACAGATCCGCCTTAGCTCCGTCGTA
TCCTTTCTTCAATATAACCTCCGGCGCAACATAAGCCGGAGTTCCACAAGTTGTATGAAG
AAGTCCGTCTTGTTTCAAATGCTCAGTGAAAGCAGAGAGACCAAAATCAGTCACCTTGAG
ATTACCTTCTTCATCTAACAAGAGATTCTCCGGTTTCAGATCTCGGTGATAAACACCACG
GCTATGACAGAAATCAACGGCTGAGATCAATTGCTGGAAATAAACACGAGCTACGTCTTC
ACGTAGTCTTCCTTGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
AACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATT
CGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11