Sequences from confirmation amplicon


Confirmation sequences for line 201G01 and hit At3g06450 are shown.
Line ID 201G01
Locus At3g06450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1977991
Primers used for PCR 42fm - 8474, show primer details
   
Sequence Name 18-K111117-0022-201-G01C-42fm
GenBank Accession LN494359 [GenBank]
Sequencing primer gene specific primer, 42fm: 5'-TAGACTTCTCCAATTGTTGCATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K111117-0022-201-G01C-42fm
TTGTTTCGAGTACTGCACATCAACCAGTTTTTAAATGCCTTTTGAGAAATACAATTGAAG
GCCTACATAAAACACACTACAATATGTAATCAAACCTGGTGATTCAGTGTTGCCAAGCTT
TTTGTGTGCATTGGAGACTGAGGGATGACACCATTGGATGGAGGAATTCCGATGAGACCG
CAAAGGATTGTCTACAAAAGAAGAGTTCCAACCTTTCATATTTCGTAACTGGTCAAGTCA
ATAAATCAGTCAAGAAAAACATACACTTCATCTCTTACCAAGAAACCTAGAAGAAACAGA
TCATAATGGTAAGCAGGAGGCTTTCTCAGATTGAAATCTTCCTGCTGTGCGAGCTGCGAG
GCTACACTATGGTCAAAGTAGTAAAGAACCGCAATCATTGACGCTGGAACAACCGCTAAA
AGAATGTAAAGCACAGGTACATCCACCATCTCCTGCAGTTTATTCATCATGCACTTTCAT
GAATATCAACATGAAGCATTAATAGTAAAGAGAATAATAAGTGTAGAGGAAGTGATTAGT
GCCTTAATGACAGTCCAATTCTGATATGCACCAGGAGACCATGGATTAGGACTAACAAGA
CGTCTTGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCG
   
Sequence Name 02-K111116-0022-201-G01C-8409
GenBank Accession LN487090 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K111116-0022-201-G01C-8409
ATCCTGGTCTCCTGGTGCATATCAGAATTGGACTGTCATTAAGGCACTAATCACTTCCTC
TACACTTATTATTCTCTTTACTATTAATGCTTCATGTTGATATTCATGAAAGTGCATGAT
GAATAAACTGCAGGAGATGGTGGATGTACCTGTGCTTTACATTCTTTTAGCGGTTGTTCC
AGCGTCAATGATTGCGGTTCTTTACTACTTTGACCATAGTGTAGCCTCGCAGCTCGCACA
GCAGGAAGATTTCAATCTGAGAAAGCCTCCTGCTTACCATTATGATCTGTTTCTTCTAGG
TTTCTTGGTAAGAGATGAAGTGTATGTTTTTCTTGACTGATTTATTGACTTGACCAGTTA
CGAAATATGAAAGGTTGGAACTCTTCTTTTGTAGACAATCCTTTGCGGTCTCATCGGAAT
TCCTCCATCCAATGGTGTCATCCCTCAGTCTCCAATGCACACAAAAAGCTTGGCAACACT
GAATCACCAGGTTTGATTACATATTGTAGTGTGTTTTATGTAGGCCTTCAATTGTATTTC
TCAAAAGGCATTTAAAAACTGGTTGATGTGCAGTTACTTCGAAACAAACTCGTGGCAGCT
GCGCGTAAATGCATCAGAAACAATGCAACATT

Last Updated on 06.12.2018 16:11