Sequences from confirmation amplicon


Confirmation sequences for line 202G01 and hit T14N5 are shown.
Line ID 202G01
Locus T14N5 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29036964
Primers used for PCR FA44 - 8474, show primer details
   
Sequence Name 23-K037322-022-202-G01C-FA44
GenBank Accession LN496469 [GenBank]
Sequencing primer gene specific primer, FA44: 5'-GGGACAATAAATAAAGAGCATCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K037322-022-202-G01C-FA44
GCCAAAAATTACAAAGGTCACATTCATCATAAATCTATTGAATTTAAGAACTTTTATGTT
GTAACCAAGATTGTGTCTTCCTTTCTACTCATTTTTCGCCCAAGAAAAACAATTATAGTA
GGTCCAAAAGTAATCATTTACCCAAACAAAACAAAAAAACGAGTTTTTCAAGACTTTCAT
GAACAAGCTAGTTAGAGAAACATCAACGTCATGCATGTTGCAAATTCGTTCATATATCTT
AGTCATTACTCATTTGTGTTACTAATGGTTTTGTATAGTGAAGAGAGTTGAATTTATCTA
CAGGCTGCATCTAGTTTTGTAAAACATAAAATTACAAAACTCCTTTACATATCTTAGATT
AACTCTTTTCTTTTTCTTTTTCTGTCATAAATCAAAATCGAGTTTTTATTTGATTGATGG
TTTATGTATGTCAAGTAGTAGTATTATATTTAATGTAGATATTTTAGACTAGCTAGTGTC
CATGGTCATTTCGTCTTAAGTTTGAATTAATGATCCATTAAATAATACACGTGTTTTATC
ATTGAAGGGTATTTGATCAATGAAATATGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
AACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATT
CGGAAATCTNTATNTCGACGTGTCTAC
   
Sequence Name 31-K037322-022-202-G01C-8409
GenBank Accession LN500072 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K037322-022-202-G01C-8409
GAGCCATTTACATATTTCATTGATCAAATACCCTTCAATGATAAAACACGTGTATTATTT
AATGGATCATTAATTCAAACTTAAGACGAAATGACCATGGACACTAGCTAGTCTAAAATA
TCTACATTAAATATAATACTACTACTTGACATACATAAACCATCAATCAAATAAAAACTC
GATTTTGATTTATGACAGAAAAAGAAAAAGAAAAGAGTTAATCTAAGATATGTAAAGGAG
TTTTGTAATTTTATGTTTTACAAAACTAGATGCAGCCTGTAGATAAATTCAACTCTCTTC
ACTATACAAAACCATTAGTAACACAAATGAGTAATGACTAAGATATATGAACGAATTTGC
AACATGCATGACGTTGATGTTTCTCTAACTAGCTTGTTCATGAAAGTCTTGAAAAACTCG
TTTTTTTGTTTTGTTTGGGTAAATGATTACTTTTGGACCTACTATAATTGTTTTTCTTGG
GCGAAAAATGAGTAGAAAGGAAGACACAATCTTGGTTACAACATAAAAGTTCTTAAATTC
AATAGATTTATGATGAATGTGACCTTTGTAATATTTTTGGCTAGTTACGTGTCACATATT
TGGATGCTCT

Last Updated on 06.12.2018 16:11