Sequences from confirmation amplicon


Confirmation sequences for line 203D09 and hit At5g09910 are shown.
Line ID 203D09
Locus At5g09910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3093484
Primers used for PCR 16oi - 8474, show primer details
   
Sequence Name 89-K114716-0022-203-D09C-16oi
GenBank Accession LN514974 [GenBank]
Sequencing primer gene specific primer, 16oi: 5'-AAGAACGTTAAGGAGGAAGAAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K114716-0022-203-D09C-16oi
GCTCTAGAATTGAAATTGATCCTCTTACTATTCCTTAGATTCGCTGCTTACCTCCATCAA
TTGTGTTAACTTCGGTATTGGTTTCGATTTGTGCTCTAGAGATTATTTGCACTAGGAAGG
TGAAGGAACTGTAGCCGAGACTCTGTTTTGCAATGTGCAAGGTGATGGATTAGAATAATC
TGCTTACAGCTAGGAATCAGAGTTTCGAAATGATGATTTCTGGAAGCTTCTCTTTTGGTC
GAAGACGCGACTATGGGACTTAATTTCGCAGTGCCGATTTTGCTGGAGTAATCAAAATGC
GGTTTTGGAGGGAACGTGGCAGGGAAAATAAGGAACATATGGTAGCGCCATTATGCGGCC
AAATTCGAGTATTGGTGGTTGGTGATTCAGGTAATATTAACAAATTCTCGTTTCCATAGA
TTTGGTAATGGTTCTAGCTTTTTAAGTAGCCATTTAATGGAACTAAAGAAAGTTTGTCGT
AGTGCACAAACTCATCCTTAAAGAAGGAGCTAATTGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATATCCGC
   
Sequence Name 41-K114715-0022-203-D09C-8409
GenBank Accession LN504178 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K114715-0022-203-D09C-8409
CTCCTTCTTTAGGATGAGTTTGTGCACTACGACAAACTTTCTTTAGTTCCATTAAATGGC
TACTTAAAAAGCTAGAACCATTACCAAATCTATGGAAACGAGAATTTGTTAATATTACCT
GAATCACCAACCACCAATACTCGAATTTGGCCGCATAATGGCGCTACCATATGTTCCTTA
TTTTCCCTGCCACGTTCCCTCCAAAACCGCATTTTGATTACTCCAGCAAAATCGGCACTG
CGAAATTAAGTCCCATAGTCGCGTCTTCGACCAAAAGAGAAGCTTCCAGAAATCATCATT
TCGAAACTCTGATTCCTAGCTGTAAGCAGATTATTCTAATCCATCACCTTGCACATTGCA
AAACAGAGTCTCGGCTACAGTTCCTTCACCTTCCTAGTGCAAATAATCTCTAGAGCACAA
ATCGAAACCAATACCGAAGTTAACACAATTGATGGAGGTAAGCAGCGAATCTAAGGAATA
TGTAAGAGGATCAATATTCGAATTCTAAGAGCGTAATAAGAGTTTCACAGAAGTAAGATT
CACCTTCTTCCTCCTAAAACGT

Last Updated on 06.12.2018 16:11