Sequences from confirmation amplicon


Confirmation sequences for line 203F04 and hit At5g52920 are shown.
Line ID 203F04
Locus At5g52920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21464036
Primers used for PCR MS20 - 8474, show primer details
   
Sequence Name 53-K016402-022-203-F04C-8409
GenBank Accession LN507983 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K016402-022-203-F04C-8409
TTCCCGGACATGAAGCCATTTACAATTGAATATATGAGCTGATGATAAATTTGTAAAATT
GAATTTCTTTTGTCTTTATGTGAGCTGATGATAAATTTGTTGTCTTACAATGTGGATTAG
AGAGAGGAGCAGCTTGAGAGGTTGTTGGAAATGCAGCAGTTTGGTGATACATCGGTAGGG
ATGTGGTCGAAGCCGACAGTGAGGAGGAAGACAAAGATTGTTTGCACCGTTGGTCCGTCG
ACCAACACACGAGAAATGATATGGAAATTGGCTGAAGCTGGGATGAATGTTGCTAGGATG
AATATGTCTCATGGAGATCATGCTTCACATAAGAAGGTTATTGATTTGGTTAAAGAATAC
AATGCACAAACTAAAGACAACACTATTGCTATCATGCTTGACACCAAGGTTTGTTTTTGT
TTCCTATTTATCTATTTACTTGAGAGTCTCTATGTCCATG
   
Sequence Name 57-K016402-022-203-F04C-MS20
GenBank Accession LN509259 [GenBank]
Sequencing primer gene specific primer, MS20: 5'-AGGCGATACATGGACATAGAGACT-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K016402-022-203-F04C-ms20
ATACATAAACAGGAAACAAAAACAAACCTTGGTGTCAAGCATGATAGCAATAGTGTTGTC
TTTAGTTTGCGCATTGTATTCTTTAACCAAATCAATAACCTCCTTATGTGAAGCATGATC
TCCATGAGACATATTCATCCTAGCAACATTCATCCCAGCTTCAGCCAATTTCCATATCAT
TTCTCGCGTGTTGGTCGACGGACCAACGGCGCAAACAATCTTTGTCTTCCCTCCTCACTG
TCGGCTTCGACCACATCCCTACCGATGTATCACCAAACTGCT

Last Updated on 06.12.2018 16:11