Sequences from confirmation amplicon


Confirmation sequences for line 203F11 and hit At2g19740 are shown.
Line ID 203F11
Locus At2g19740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8514277
Primers used for PCR 02vy - 8474, show primer details
   
Sequence Name 44-K118356-0022-203-F11C-02vy
GenBank Accession LN505333 [GenBank]
Sequencing primer gene specific primer, 02vy: 5'-AAACCAATTCGTTACACTTCAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K118356-0022-203-F11C-02vy
GAAGATGATTGCGAAGTAAAGTGCGTCACAAAAAGATCAAACTCGTATGAGTAGTACCAA
ATCTGTTGTCTATAGAACTTAAAACATAAGAAATGATAAATGACCAAAATAGGTAAGTGA
TCAAAACAAGCAAAGTCTTTTCGTTTCGTCTTTTCATCTAAAGATAAGGTTGTGTGAGTG
TACATTCACCAATTTTCACTCCTCTTCTTCAATGACCTTAGTACCCAAACCAGATAATCC
TTCAGCCGGAATCTCAGCGACGGTGCATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 44-K118355-0022-203-F11C-8409
GenBank Accession LN505332 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K118355-0022-203-F11C-8409
GGTACAAGGGTCATTGAAGAAGAGGAGTGAAAATTGGTGAATGTACACTCACACAACCTT
ATCTTTAGATGAAAAGACGAAACGAAAAGACTTTGCTTGTTTTGATCACTTACCTATTTT
GGTCATTTATCATTTCTTATGTTTTAAGTTCTATAGACAACAGATTTGGTACTACTCATA
CGAGTTTGATCTTTTTGTGACGCACTTTACTTCGCAATCATCTTTCTGATTCTGTACCAG
ACCGGTTTATTGTCTGGGTTGAAGTGTACNAAATTGGTTT

Last Updated on 06.12.2018 16:11