Sequences from confirmation amplicon


Confirmation sequences for line 203G01 and hit At3g55430 are shown.
Line ID 203G01
Locus At3g55430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20551164
Primers used for PCR 11fm - 8474, show primer details
   
Sequence Name 22-K111117-0022-203-G01C-11fm
GenBank Accession LN496167 [GenBank]
Sequencing primer gene specific primer, 11fm: 5'-GTAACCTGAGATCAATTGCAACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K111117-0022-203-G01C-11fm
ACTACCATCTCCACAATTTTCATATGATAATCATGTTGGTCTAAATTTCCTATGAAATTG
GTACATTATCTCATTTACCTAGGATCACATATATCATGATGTAATAAAGGAAACATAAGG
CATGGCAGACAGATGAACGAATCTAAGGACAATAGGATAACCTTGGACGGTGATGAGATA
AGCACGTGGGATAGTCTAAATGGTCAGATCTTAATCCAATCGCTAACATTTTCAAGTTTC
AAATCTTTGAATTTTGCTTTGGGAATTTATTTTTTTCCTCTTTTTAAGAAACTAACAATA
TACAAGTTGGATGAAAAAAAGAAAGAAGCAAACGCAACATTCTACGTTTGCGTGAAATTT
TGGATTTCAAGTTAGATAACAGACGAAAAAATAGAGATTGAATCTAATTTTTCAAAATAA
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CC
   
Sequence Name 06-K111116-0022-203-G01C-8409
GenBank Accession LN488869 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K111116-0022-203-G01C-8409
CATTGATTATTTTGAAAATTAGATTCAATCTCTATTTTTTCGTCTGTTATCTAACTTGAA
ATCCAAAATTTCACGCAAACGTAGAATGTTGCGTTTGCTTCTTTCTTTTTTTCATCCAAC
TTGTATATTGTTAGTTTCTTAAAAAGAGGAAAAAAATAAATTCCCAAAGCAAAATTCAAA
GATTTGAAACTTGAAAATGTTAGCGATTGGATTAAGATCTGACCATTTAGACTATCCCAC
GTGCTTATCTCATCACCGTCCAAGGTTATCCTATTGTCCTTAGATTCGTTCATCTGTCTG
CCATGCCTTATGTTTCCTTTATTACATCATGATATATGTGATCCTAGGTAAATGAGATAA
TGTACCAATTTCATAGGAAATTTAGACCAACATGATTATCATATGAAAATTGTTGGAGAT
TGGTAGGTAACAATTGATTCTAATACTACTATTTGTTGCAATTGA

Last Updated on 06.12.2018 16:11