Sequences from confirmation amplicon


Confirmation sequences for line 204A04 and hit At3g10740 are shown.
Line ID 204A04
Locus At3g10740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3362471
Primers used for PCR IF26 - 8474, show primer details
   
Sequence Name 13-K032413-022-204-A04C-8409
GenBank Accession LN491923 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K032413-022-204-A04C-8409
CGGAATGAGCATTTACAATTAATACTCATTGGAAAACTTACTACAGAGGTTTTGCATTTC
TCTATGATCACATCTTGACTTAGATTTTTGTTGAGCATCTTTGACACTAAATGAGATTAC
AACTTTACAGGAAACTACATTGTGTTCTATGATGCTATCAAGAAAGCCTATCCAGATATC
AAAATCATCTCCAACTGCGATGGATCCTCTCATCCACTCGACCACCCAGCTGATTACTAT
GACTATCACGTAAAATTTTCTATCTTGACAGAAACTGTAACCGTTGTCTTGTGTTCTCTC
TTCCGCCTGAAAAAAGTGTTTCCTTTTTGTCACAGATTTACACTTCTGCTAGCAATTTGT
TTTCCATGTATCATCAATTTGATCGCACTTCACGCAAAGGTCCAAAGGTGCGGTAATAGA
TTATAACAAAACTTAATTCCAACAAACACAAGATGAAGCTTTCAGCGTTTTACTCACTCG
TGAACTTTGTTTACAGGCTTTTGTCAGTGAATATGCTGTTACTGGAAAAGATGCTGGTAC
AGGAAGCCTTCTTGCCTCTCTTGCAGAAGCTGCGTTTCTCATTGGTCTTGAAAAAATTAG
GTTCC
   
Sequence Name 13-K032413-022-204-A04C-IF26
GenBank Accession LN491924 [GenBank]
Sequencing primer gene specific primer, IF26: 5'-GGAACCTATTCTTTTCAAGACCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K032413-022-204-A04C-IF26
CAGAAGGCTTCCTGTACAGCATCTTTTCAGTAACAGCATATTCACCGACAAAAGCCTGTA
AACAAAGTTCACGAGTGAGTAAAACGCTGAAAGCTTCATCTTGTGTTTGTTGGAATTAAG
TTTTGTTATAATCTATTACCGCACCTTTGGACCTTTGCGTGAAGTGCGATCAAATTGATG
ATACATGGAAAACAAATTGCTAGCAGAAGTGTAAATCTGTGACAAAAAGGAAACACTTTT
TTCAGGCGGAAGAGAGAACACAAGACAACGGTTACAGTTTCTGTCAAGATAGAAAATTTT
ACGTGATAGTCATAGTAATCAGCTGGGTGGTCGAGTGGATGAGAGGATCCATCGCAGTTG
GAGATGATTTTGATATCTGGATAGGCTTTCTTGATAGCATCATAGAACACAATGTAGTTT
CCTGTAAAGTTGTAATCTCATTTAGTGTCAAAGATGCTCAACAAAAATCTAAGTCAAGAT
GTGATCATAGAGAAATGCAAAACCTCTGTAGTAAGTTTTCCAATGAGTATTTAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTT
ATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGG
CGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT
Primers used for PCR 69wv - 8474, show primer details
   
Sequence Name 63-K118684-0022-204-A04C-69wv
GenBank Accession LN511091 [GenBank]
Sequencing primer gene specific primer, 69wv: 5'-TGTAGAGCCTTGAGATGGAAGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K118684-0022-204-A04C-69wv
TGGAGAGACTCTATCAAATACTCGATCTTTTTGGTTGATTGCAGGGACATGGTTTTAGAA
ACGATCTTTTCCAAATGATGGCCGATATAAAACCCCGTTTCATCCGGTTCCCAGGTACGT
ATTATTTAAGGCTAGTAGCATTTTTGTTACTTATTCTATCCATAGACGTACCTCTTAGTT
CAATATGACAAAAAGCATGACTTAAGATGAAATAAGCTAAAGTCTATTCTTTACTCTGGA
TATTTGTGAATTTAGGTGGTTGTTTTGTCGAGGGTGAATGGTTAAGTAATGCCTTCCGCT
GGAAAGAAACCGTAGGACCTTGGGAAGAGAGACCGGGCCATTTTGGTGATGTTTGGAAGT
ATTGGACCGATGATGGCCTTGGCCACTTTGAGTTCTTTCAAGTGAGACTTCTTCTTTTCT
ATTGAGTACATAATGATCCCCGAAGCTCGTTATTGAATATTAAATGAACATTCTTGCAGA
TGGCAGAAGACATCGGTGCAGCCCCGATATGGGTGTTTAACAACGGTATTCTTTCACTCT
CCAAAAACTTAGTTGTAACTGACTTATATCATTTTGTCTTCCAATTCTGTCAACCTTATT
TTTTACTTTGTGCAGGGATAAGTCACAATGATGAAGTTGAAACTGCCAGTATCATGCCCT
TTGTTCAAGTATGATTACTAAACCTTCCTCTGTTTCACTAGTGAAGAAGCCGGAAAACCA
AAACTTTCTCCTCTTTTTGTTTCAGGAAGCACTCGACGGTATCGAGTTTGCCCGAGGAGA
TGCCAATTCTACTTGGGGATCTGTTCGAGCTAAAATGGGACGCCAAGAACCTTTTGAACT
TAATACGTTGCTATTGGAAATGAAGATTGTGGCTTCATGTCAATATGGAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
   
Sequence Name 51-K118683-0022-204-A04C-8409
GenBank Accession LN507647 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K118683-0022-204-A04C-8409
ATTTCCTATTGACTGAAGCCACAATCTTCATTTCCAATAGCAACGTATTTAAGTTCAAAA
GGTTCTTGGCGTCCCATTTTAGCTCGAACAGATCCCCAAGTAGAATTGGCATCTCCTCGG
GCAAACTCGATACCGTCGAGTGCTTCCTGAAACAAAAAGAGGAGAAAGTTTTGGTTTTCC
GGCTTCTTCACTAGTGAAACAGAGGAAGGTTTAGTAATCATACTTGAACAAAGGGCATGA
TACTGGCAGTTTCAACTTCATCATTGTGACTTATCCCTGCACAAAGTAAAAAATAAGGTT
GACAGAATTGGAAGACAAAATGATATAAGTCAGTTACAACTAAGTTTTTGGAGAGTGAAA
GAATACCGTTGTTAAACACCCATATCGGGGCTGCACCGATGTCTTCTGCCATCTGCAAGA
ATGTTCATTTAATATTCAATAACGAGCTTCGGGGATCATTATGTACTCAATAGAAAAGAA
GAAGTCTCACTTGAAAGAACTCAAAGTGGCCAAGGCCATCATCGGTCCAATACTTCCAAA
CATCACCAAAATGGCCCGGTCTCTCTTCCCAAGGTCCTACGGTTTCTTTCCAGCGGAAGG
CATTACTTAACCATTCACCCTCGACAAAACAACCACCTAAATTCACAAATATCCAGAGTA
AAGAATAGACTTTAGCTTATTTCATCTTAAGTCATGCTTTTTGTCATATTGAACTAAGAG
GTACGTCTATGGATAGAATAAGTAACAAAAATGCTACTAGCCTTAAATAATACGTACCTG
GGAACCGGATGAAACGGGGTTTTATATCGGCCATCATTTGGAAAAGATCGTTTCTAAAAC
CATGTCCCTGCAATCAACCAAAAAGATCGAGTATTTGATAGAGTTCTTCTTCCAATATTT
TCTCTATTAGCAATCTGGTATCCTCCATCT

Last Updated on 06.12.2018 16:11