Sequences from confirmation amplicon


Confirmation sequences for line 204B06 and hit At5g42460 are shown.
Line ID 204B06
Locus At5g42460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16979785
Primers used for PCR JY06 - 8474, show primer details
   
Sequence Name 14-K024506-022-204-B06C-JY06
GenBank Accession LN492336 [GenBank]
Sequencing primer gene specific primer, JY06: 5'-TCTCAGGATTTTGTGGTTCTTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K024506-022-204-B06C-JY06
CTCATCGTACCCAAGAGCGTAATTGTCCTTTCTGTGGTAAGAATTTCTGGGTTGGATCCA
TCTTGTTTGACCAAAATAAGGGTTCCAAACCACAAGCCTATAGTGGAGGTCTTTGGTGAT
GCATAATAGTAAACCATCGCAGTGAAAGACTCTAGATACATTATCGACTTGATGATGGTT
TGCATCATCGCTTAAGCTAATAAGTGTACCTTTAGGCGTAAATGATGGATCAACATTGTT
GTGGATATCGATGCTTGTTAGATAAACCCTAAAATTCCTCATCATGATCACCTCAATCTC
CTTCGATTCCAATTGCTTCTTCTTTGTTTCGACGATTTGCTTCTTGAGAAAGCTCCGATC
TTTGGATAAATCGTTCCACTTTTTGCAAGTCAATCGCACTGCTCTTAGAGATGTCAACGG
AACCCTACTGAGAATCTCCGCTAACAAATCCCGTGGAAGATCTGAAACGATGTTTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
CGTTATTATAAAATGAAAGTACATTTTGATAAAACGACACAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA
   
Sequence Name 13-K024506-022-204-B06C-8409
GenBank Accession LN491866 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K024506-022-204-B06C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAAACATCGTTTCAGATCTTCCACGGG
ATTTGTTAGCGGAGATTCTCAGTAGGGTTCCGTTGACATCTCTAAGAGCAGTGCGATTGA
CTTGCAAAAAGTGGAACGATTTATCCAAAGATCGGAGCTTTCTCAAGAAGCAAATCGTCG
AAACAAAGAAGAAGCAATTGGAATCGAAGGAGATTGAGGTGATCATGATGAGGAATTTTA
GGGTTTATCTAGCAAGCATCGATATCCACAACAATGTTGATCCATCATTTACGCCTAAAG
GTACACTTATTAGCTTAAGCGATGATGCAAACCATCATCAAGTCGATAATGTATCTAGAG
TCTTTCACTGCGATGGTTTACTATTATGCATCACCAAAGACCTCCACTATAGGCTTGTGG
TTTGGAACCCTTATTTTGGTCAAACAAGATGGATCCAACCCAGAAATTCTTACCACAGAA
AGGACAATTACGCTCTTGGGTACGATGAGAAGAAGAACCAC

Last Updated on 06.12.2018 16:11