Sequences from confirmation amplicon


Confirmation sequences for line 204C09 and hit At3g27835 are shown.
Line ID 204C09
Locus At3g27835 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10321923
Primers used for PCR 81we - 8474, show primer details
   
Sequence Name 75-K118393-0022-204-C09C-81we
GenBank Accession LN513302 [GenBank]
Sequencing primer gene specific primer, 81we: 5'-GTTGCTCTTCTTGCCCCAAGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K118393-0022-204-C09C-81we
ATTTTTTCTGTTTATATATCTCAAATAGCAAAATTCATGAGTTAACACTAACTAGCAAAC
CAAATGTTTATTTTATATCAAATTTTGTATCATATCTCCACACATATAGTTAAAGATATT
GTATACACTTTATTTTCACAATACATTGTGATATTAAGCTTTTACAAATAAAAAATACAT
TGTGATATTGCGTTGTAACCCATTGAAAGAGATTCTTGTCGAGTGATTTTTTTTTTTTTT
TTGCCCTTTTTTTTCCAAAATTTTTTTTTTAAATTTAAAAAAATTTTTGGAAAAACAAAG
GGGAAAAACCCCCCAAAATCTTGGGCAAAATTAACCCCTCCCCAAAAAGGGAACTTTGCC
AAAGGTTTTTCCCAAAATTTCCGGAACGGAAAAAACCCCGGAAAAAAAAAAAATTTCCCG
GGGGAAACTTTCCCCTTTTAAAACCAATAAAAACAAAGGGGAAAAACCATTTGAAAAGGG
TTTCTTTCCGGGAAATTCCCCTGGGTCCCCCAAGGATTTTAAGGGCCAATTGGGAAAAAA
AAAAAAAGAATTTCCCATTTTTTTTCCATTTAAAAAAGGAAAATTCCCCC
   
Sequence Name 27-K118392-0022-204-C09C-8409
GenBank Accession LN498598 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K118392-0022-204-C09C-8409
TTTACATTGTATTTACCTATTGTTCATATTGATTTAATACGAGACATGTTCCACCTGGAA
ATTTTTGTTCTTTGCAGTGCTTATTGCAGTCCGGAAATTTTGAGCATAGACCTTCGACAA
TGTACCATTTGTGAGCAGCGTTAACTTCGACCATAGATTTCGCGTGTACTTCACCTATTG
TTTTCCAAAAATTATTGTAATATTAAAAAAAAAATTGTTGAACATAAAAGGCAAAAAAAA
AAAAAAAATCCCCCCAAAAAAATTCTTTTTAAGGGGTTAACACCCCATTACCCAAGGGTT
TTTTTTTTTTGAAAAACCTAAATTCCCAAGGGTTTGGGAAAAAAAAGGGGAAACCAAATT
TTTAAATTTTTTGGGGGGAAATTTAAAACAAATTTTGATAAAAAAAAAACTTTGGTTTGG
CTATTTGGGGTAACCCCAAAATTTTGGTTTTTTGGAAATATATTTAACCAAAAAAAATTT
AAACTTTTTTTTGGGCATTTTGGGGCAAAAAAAAACAAAA

Last Updated on 06.12.2018 16:11