Sequences from confirmation amplicon


Confirmation sequences for line 204G11 and hit At3g50110 are shown.
Line ID 204G11
Locus At3g50110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18583620
Primers used for PCR 05gg - 8474, show primer details
   
Sequence Name 73-K111813-0022-204-G11C-8409
GenBank Accession LN512924 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K111813-0022-204-G11C-8409
ATTGAATATATCCTGGGCCAAAAGTTTTAAATTCCAGCAGCAAGATCCAAATCGTACAGA
TTCTGGGATGTCAGCTTTCACAAGATTCACCTCTGAACTTGGTCTGCATTTGCCAACGAA
AGGGTCTGAGGAGGTTGGTGATTCTAGATCATCAAACACACAAGTTGGTGGTGCTTTTGA
GTCCCTCACAAAAGCTGTGGTTGATTCATCTCGTGGTGCCGTTAAAGCAATGCAGGTGAA
AGCACGCCACATTGTCTCTCAGAACAAAAGAAGATACCAGGTATGGTAATGCTGTCTTTC
TGCTTTTGTTAGATTCTTTGAAACTANGGCACCTT
   
Sequence Name 81-K111814-0022-204-G11C-05gg
GenBank Accession LN514086 [GenBank]
Sequencing primer gene specific primer, 05gg: 5'-TAAGGTGCCTCAGTTTCAAAGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K111814-0022-204-G11C-05gg
ATCTTCTTTTGTTCTGAGAGACAATGTGGCGTGCTTTCACCTGCATTGCTTTAACGGCAC
CACGAGATGAATCAACCACANGCTTTTGTGAGGGACTCAAAAGCACCACCAACTTGTGTG
TTTGATGATCTAGAATCACCAACCTCCTCAGACCCTTTCGTTGGCAAATGCAGACCAAGT
TCAGAGGTGAATCTTGTGAAAGCTGACATCCCAGAATCTGTACGATTTGGATCTTGCTGC
TGGAATTTAAAACTTTTGGCCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCAGCACCGATTATT

Last Updated on 06.12.2018 16:11