Sequences from confirmation amplicon


Confirmation sequences for line 205C02 and hit At2g26560 are shown.
Line ID 205C02
Locus At2g26560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11295389
Primers used for PCR EO05 - 8474, show primer details
   
Sequence Name 63-K022563-022-205-C02C-8409
GenBank Accession LN510884 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K022563-022-205-C02C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGTAAATGGGATTAGGCGCGGTGAGCATGG
CTGTCACTAGACCACCGGTGCTTGTTCCCGCTATTACATCAAAGTAGTCTGCAAGCCTTG
CTTCTTCTCCATCCAATTTCTATGCATCCATGTATATATATGAGTTAAGAAAATATAATT
TTACAAACATAATATTTGTATAGTTAGGTAGCACATATATACCTGGAGTTCGGACTCGAG
AAAACCAAGGATAACGGCAGGGATTAGCCCTCTAATGCCACCACCGTCGATGCTGAGGAT
TGTAACTAAGTTTCCATAGGTCGGGGGCTGGAGAGGAGATTTGGGGCTGTCCATTTGCAT
CTTA
   
Sequence Name 64-K022563-022-205-C02C-EO05
GenBank Accession LN511104 [GenBank]
Sequencing primer gene specific primer, EO05: 5'-CTTGTTCTGTAAGATGCAAATGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K022563-022-205-C02C-EO05
CAGCCCCAAATCTCCTCTCCAGCCCCCGACCTATGGAAACTTAGTTACAATCCTCAGCAT
CGACGGTGGTGGCATTAGAGGGCTAATCCCTGCCGTTATCCTTGGTTTTCTCGAGTCCGA
ACTCCAGGTATATATGTGCTACCTAACTATACAAATATTATGTTTGTAAAATTATATTTT
CTTAACTCATATATATACATGGATGCATAGAAATTGGATGGAGAAGAAGCAAGGCTTGCA
GACTACTTTGATGTAATAGCGGGAACAAGCACCGGTGGTCTAGTGACAGCCATGCTCACC
GCGCCTAATCCCATTTACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11