Sequences from confirmation amplicon


Confirmation sequences for line 205C10 and hit At2g47510 are shown.
Line ID 205C10
Locus At2g47510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19500644
Primers used for PCR CP06 - 8474, show primer details
   
Sequence Name 08-K031007-022-205-C10C-8409
GenBank Accession LN489614 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K031007-022-205-C10C-8409
GCCATTATTCCTAAACGGTGAAATCAGTTGCTAAATTATTCTGCTTTTTCCAGGCTGCAC
ACGATGCTTGTGTTGAAACCAGTGGGTCACTTAACACAATCGCCACATCGCTGATGAAGA
TCGCCAATGATATACGTTTTCTTGGAAGGTATGGATCTAATCTATAGGGATTATTTTTTT
CTTTCTTTAACATGCACTATGTCCTTATGGTTTACCATGTCTTTATATAGTGGCCCAAGA
TGTGGTCTTGGTGAACTTGTACTACCTGAAAATGAGCCAGGAAGCAGTATCATGCCTGTA
TGAATGCAATCTTCCTTTGCTCTTCTGTCCTTTTCTTTTTCACTAGTTTTGATTCAAATG
AATTTTTATCTCTTTCTCAGGGGAAGGTAAATCCTACACAGTGTGAGGCCTTGACTATGG
TTTGTGCACAGGTAAGTCCCATCTATCAATACATTCTTAGTACAGACCAATGAATCTAGA
TGCTAAGGTTGTATTATTGTTCCTTGACTGTTTGGGTTGCAACCAATAACATTGCAAGGT
TAT
   
Sequence Name 09-K031007-022-205-C10C-CP06
GenBank Accession LN490056 [GenBank]
Sequencing primer gene specific primer, CP06: 5'-CATAACCTGCAATGTTATTGGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K031007-022-205-C10C-CP06
ATAATCAACCTTAGCATCTAGATTCATTGGGTCTGTACTAAGAATGTATTGATAGATGGG
ACTTACCTGTGCACAAACCATAGTCAAGGCCTCACACTGTGTAGGATTTACCTTCCCCTG
AGAAAGAGATAAAAATTCATTTGAATCAAAACTAGTGAAAAAGAAAAGGACAGAAGAGCA
AAGGAAGATTGCATTCATACAGGCATGATACTGCTTCCTGGCTCATTTTCAGGTAGTACA
AGTTCACCAAGACCACATCTTGGGCCACTATATAAAGACATGGTAAACCATAAGGACATA
GTGCATGTTAAAGAAAGAAAAAAATAATCCCTATAGATTAGATCCATACCTTCCAAGAAA
ACGTATATCATTGGCGATCTTCATCAGCGATGTGGCGATTGTGTTAAGTGACCCACTGGT
TTCAACACAAGCATCGTGTGCAGCCTGGAAAAAGCAGAATAATTTAGCAACTGATTTCAC
CGTTTAGGAATAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGT
CGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACG
TGTCTACATTCA

Last Updated on 06.12.2018 16:11