Sequences from confirmation amplicon


Confirmation sequences for line 205D04 and hit At3g24130 are shown.
Line ID 205D04
Locus At3g24130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8711904
Primers used for PCR BL94 - 8474, show primer details
   
Sequence Name 55-K105140-0022-205-D04C-BL94
GenBank Accession LN508807 [GenBank]
Sequencing primer gene specific primer, BL94: 5'-GGCCAAGGAATTGTTAACAGTAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K105140-0022-205-D04C-BL94
ATATATCTTTCTACCACTGCGATTGGAATGTTTTTTTCACTTTTCCTATGCACAAAAAGG
AAAATATCGAACTGAAGTTTAATTTTATGAAAAGTAAAGATATGATAAATTAAAAATTGA
ACCAAACTAAGAATGTTCTTGATTGGATTTTGATTTGATAACCATTTTCGAAAAAAAACT
AAATATACATAGCTAATGGAACCATTCTTAATAGCCCTGTAGTTTTCACATATTAATAAT
CCTTTATAAAACTAAAATTCCTATTTATTCGGGCCAAGTTTCATTAAATAAAAGTTGTGT
TTGGTGGGTTGTAGAGCTGTGTGATACAAGTGCTAGGTGGGCAGCTAGAACCGGGGCTAG
CCGGTTACATAACGGCTCAAGGACGGACCAACCCGTACGATGCAAATGGATTCATCTTCA
TCAACTGCCTCGTATATGGAACAGGCATGGCCTTCTTGGGCAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 39-K105141-0022-205-D04C-8409
GenBank Accession LN503348 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K105141-0022-205-D04C-8409
TTACAATTGAATAATCCTGCCCAAGAAGGCCATGCCTGTTCCATATACGAGGCAGTTGAT
GAAGATGAATCCATTTGCATCGTACGGGTTGGTCCGTCCTTGAGCCGTTATGTAACCGGC
TAGCCCCGGTTCTAGCTGCCCACCTAGCACTTGTATCACACAGCTCTACAACCCACCAAA
CACAACTTTTATTTAATGAAACTTGGCCCGAATAAATAGGAATTTTAGTTTTATAAAGGA
TTATTAATATGTGAAAACTACAGGGCTATTAAGAATGGTTCCATTAGCTATGTATATTTA
GTTTTTTTTCGAAAATGGTTATCAAATCAAAATCCAATCAAGAACATTCTTAGTTTGGTT
CAATTTTTAATTTATCATATCTTTACTTTTCATAAAATTAAACTTCAGTTCGATATTTTC
CTTTTTGTGCATAGGAAAAGTGAAAAAAACATTCCAATCGCAGTGGTTAGAAAGATATAT
CTAGATTGAACATCACAACTATCTACGGACATCTACTGTT
Primers used for PCR BL93 - 8474, show primer details
   
Sequence Name 56-K105140-0022-205-D04C-BL93
GenBank Accession LN509097 [GenBank]
Sequencing primer gene specific primer, BL93: 5'-CAAACAAAACGATGCACTACAGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K105140-0022-205-D04C-BL93
ACTTGTGTTTACACACCTGTCACAAATGTACCAGCATCCGCTTTTGTTAAACACATTCAG
AGAAAACACAAAAGTTGAAATATAAAAACAAGTTCTCTGAAAGGTTTGTTATATATTTTT
AATGTCAATGTCAAAGCTAAAAGAACATAAAGCATAACAAATTACTACATTTGGTTAAAC
TAAATAAAGAGGGATGATCAATAAGAAATCTATAAAAAATACAAAAATTAGAAGCAAAGG
AAAGATCAAGCAGGGATGGGCAAATCTTCAACCCATCCACCACGATTAATAAAGCTGAGA
TCAGCCAAATTTTGAATGGCAGATTCACTAAGCTTCTTAACCCACTTCACACGTCTTCCT
ATATTTGCTCCACTTCCAAAGCATCCATGTTCTGCAAAGACCAGCTGGTTTTCGTGGCCC
ACGAAATTCCATGCATCCCATCCTTCAGGAACAACTACATCGGTCAGGTTCGAGTTGTAA
AAGATCAGCAATGAGTATATATTCAATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATTC
   
Sequence Name 40-K105141-0022-205-D04C-OriL
GenBank Accession LN503703 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K105141-0022-205-D04C-OriL
GCCTTTCATTGAATTGAATATATACTCATTGCTGATCTTTTACAACTCGAACCTGACCGA
TGTAGTTGTTCCTGAAGGATGGGATGCATGGAATTTCGTGGGCCACGAAAACCAGCTGGT
CTTTGCAGAACATGGATGCTTTGGAAGTGGAGCAAATATAGGAAGACGTGTGAAGTGGGT
TAAGAAGCTTAGTGAATCTGCCATTCAAAATTTGGCTGATCTCAGCTTTATTAATCGTGG
TGGATGGGTTGAAGATTTGCCCATCCCTGCTTGATCTTTCCTTTGCTTCTAATTTTTGTA
TTTTTTATAGATTTCTTATTGATCATCCCTCTTTATTTAGTTTAACCAAATGTAGTAATT
TGTTATGCTTTATGTTCTTTTAGCTTTGACATTGACATTAAAAATATATAACAAACCTTT
CAGAGAACTTGTTTTTATATTTCAACTTTTGTGTTTTCTCTGAATGTGTTTAACAAAAGC
GGATGCTGGTACATTTGTGACAGGTGTTGTAAAACACAAGTTTTGTAAAAATCATATTAC
ACCGCCATAACTGTAGTG

Last Updated on 06.12.2018 16:11