Sequences from confirmation amplicon


Confirmation sequences for line 205E08 and hit At1g24267 are shown.
Line ID 205E08
Locus At1g24267 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8605095
Primers used for PCR 26oi - 8474, show primer details
   
Sequence Name 61-K114864-0022-205-E08C-26oi
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 26oi: 5'-CAAAATCTTGCTGGAAACAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K114864-0022-205-E08C-26oi
TGATGTTTCTTCGGCAATACCGCCCATACCGGCACCACCAATGCCTACTACCTGTAGGTG
TCCGCCTCTTTTTCATACCAAAAAAAAAAGGGAAAATTAAAACCGGCCTTCCAAACCCTT
CGCCAACCTCTCTGTATAACCGGAATTCCAAAGGGGCAAACCACCATCCGGATTATTTGG
GCCATTTAACCCATTTAAATATAACCGGCCCCCCTCCAAAATTTTGGCCCCTTTTTTCAC
TTTGTCTCCCCTAACCAGGGGATCAGCCAAAACCCCCCCAGGATCTTTTCAAATCCCCTC
CAACCATTTAACCTTCTTGGGCTGGCTAATCATTTAAAGGACCAACCTCTCTCTTCATTT
TTGACAAACAACTTTGGGGGTCCAAGGGATTTATTAGGCCCTGTTTTGACCCCCCTTCGA
GCCCCCCCTTTTCTGGGTTTTGAAAAAATACTAAACGGGCCAAAACTCGGGTTTCCCGTA
TTTTCCCCAAAAGGTGTCCCTTTTTCCCTTATCCCCGGAAACCCAATTTTATAAAAAATA
TTTCCGGGTTAGGTTTGACCCAACCGGATTTTTGAAACTTGGGGGAAAGGGAAAAGGGAA
ATAATTTTTCCCTTCAACCCAAACTTAATTGGAGTGTGCTAAATTGAACCACTTGCCTGC
CCAAATGTAAGGTCATTTTCCACTCAACCCAGTTTCATTTTCTAATCCAAAATTCTGTTT
GGAAATAAAACAACCCTGTGGACCTCCTTTAAAGTTTA
   
Sequence Name 21-K114863-0022-205-E08C-8409
GenBank Accession LN495775 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K114863-0022-205-E08C-8409
CGACTATCTACATTTATCTTATTTACCCCAACTTCTATTGTGTATAGGCTTTACCATCAA
CTTCAGCAGTGCCAGCCCTTGAGGCAGCTCCTATGACACCATCGTCAAGGGTAAATGACT
AAACTGGTCTCACAATGATTTTCAACTGTTTATCACGTTATTGGGATTGTTACCTCTTGT
TCCGTTCTATTGCTTATTCCTCTGTGGTGTTTTAATGTTAGCAGACTTTGTCTTTGCCTC
CTGCTTCTCCCCGTGAATCTCAGTCGCCTTCAACTTCTAATGGAGCTCAACAGGTTTGTT
CTATCTCAAACTAGAGTTTTGGATTAGAAACTGAACTTGGTTTGAGTGAATACTGACATT
ACATTTGGGCAGTCACGTGGTCCACTTCAGCACACTCAATCTATGTCTGGTTTGAAGGTA
AATATTATTTCCCTCTTCCCTTTCCCTCAAGTCTCAAACATCAGTATGGGTCAAACCTAA
CCTGAAACTATCTTTTATAAACTTGGGTCTCAGGAGATAAGCGAAAATGGAACACATTCT
GGAGAAACTACGGGAAACACGAGTTCTGGCCTGTTTAGTATTTTCTCAATACCAAGAATA
GGGAGGACTCGAAGTGTGGTCAACACAGTGCCTACTAACTCCATTGGACCCCAATAGTCG
TCTGTCAATACTGAAGAGAGAGTTTGATCATTCAAATGATCAGCAAGCCAGAGATGGTTA
AGTGCTAGTAGAGGATTTGAATAGATCATGCTGCGTCTATGCTTGATCCCTTGCTTAGTG
TAGACAATGTGATAAATGGTGCATAATTTTTGTAGGGATGCAGCTTATATTTAATTGGAT
TATATGTCACAATTAATCAGTATGTTGCTCTGCCCTTTCGTAATTCAGTTTATACAGAGA
GGTCGACGATGGTTTTGTATGGCCGTCTCTACTTTTCCNTCTTTTTTTGTATTGAATAAA
AGGCTGGTCCAT

Last Updated on 06.12.2018 16:11