Sequences from confirmation amplicon


Confirmation sequences for line 206E03 and hit At5g61130 are shown.
Line ID 206E03
Locus At5g61130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24587718
Primers used for PCR EP39 - 8474, show primer details
   
Sequence Name 48-K103737-0022-206-E03C-EP39
GenBank Accession LN506487 [GenBank]
Sequencing primer gene specific primer, EP39: 5'-GTCCTCGAAACGATAACTAAGTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K103737-0022-206-E03C-EP39
TTACATCCTTAAGCTCAGGAAAGAGCAGAATCCACTCGAAGCGATCAAGACAAGGCAGAT
GAACAATTTGCTTGAGTTCTTGAGAGCAAACGCGCTGCTGTCTGTCGTGTAATCCGGGTT
AATCCCTGTCCCAGTGGTGCCTCCTGTGCTATTTCCCCCAAAAACTCCATTGGTTGGGTT
CCCTGAATAAGGACTGTTGGTACCACTACCAGGAAGTGTGGTGGTCGTTGGGCTGCCTTT
TGGATTGGTTGTGCCAGGTGTCACAGTTGTGCTGCCGCTAGAGCCACAGGATATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
ACGNTTATT
   
Sequence Name 64-K103738-0022-206-E03C-8409
GenBank Accession LN511187 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K103738-0022-206-E03C-8409
TTTACAATTGAATAATCCTGTGGCTCTAGCGGCAGCACAACTGTGACACCTGGCACAACC
AATCCAAAAGGCAGCCCAACGACCACCACACTTCCTGGTAGTGGTACCAACAGTCCTTAT
TCAGGGAACCCAACCAATGGAGTTTTTGGGGGAAATAGCACAGGAGGCACCACTGGGACA
GGGATTAACCCGGATTACACGACAGACAGCAGCGCGTTTGCTCTCAAGAACTCAAGCAAA
TTGTTCATCTGCCTTCTCTTGATCGCTTCGAGTGGATTCTGCTCTTTCCTGATGCTCTAA
GGATGTTAATGGTCTCTGGTTCTGTGGGTGCACTTAGTTATCGTNTCGAGGA

Last Updated on 06.12.2018 16:11