Sequences from confirmation amplicon


Confirmation sequences for line 206F03 and hit At4g13260 are shown.
Line ID 206F03
Locus At4g13260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7723242
Primers used for PCR 11lx - 8474, show primer details
   
Sequence Name 42-K114144-0022-206-F03C-11lx
GenBank Accession LN504545 [GenBank]
Sequencing primer gene specific primer, 11lx: 5'-CACGGGTTTAAAAACCTAATCTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K114144-0022-206-F03C-11lx
TTTTAGATCTATTTCTCTCTTTTTTCTTCTCTTTCTCTCTTTCATGGAGTTTGTTACAGA
AACGTTAGGCAAGAGAATCCATGATCCGTACGTGGAGGAAACTAGGTGCTTAATGATTCC
CGGACCAATCATTGTCGGTTCCGGGCCGTCGGGACTGGCCACAGCGGCATGTTTAAAGTC
GAGAGACATCCCTAGTTTGATTCTAGAACGTTCCACTTGCATAGCGTCACTATGGCAGCA
CAAAACATATGATCGTCTTCGACTTCATCTCCCTAAAGATTTCTGTGAGCTTCCATTGAT
GCCTTTTCCTTCAAGCTACCCTACTTACCCTACAAAGCAACAGTTCGTCCAATACCTTGA
GTCTTACGCCGAACATTTTGACCTAAAGCCCGTTTTTAACCAGACCGTGGAGGAAGCCGG
GAAATCTACATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 15-K114143-0022-206-F03C-8409
GenBank Accession LN493063 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K114143-0022-206-F03C-8409
ATTTCCCGGCTTCCTCCACGGTCTGGTTAAAACGGGCTTTAGGTCAAAATGTTCGGCGTA
AGACTCAAGGTATTGGACGAACTGTTGCTTTGTAGGGTAAGTAGGGTAGCTTGAAGGAAA
AGGCATCAATGGAAGCTCACAGAAATCTTTAGGGAGATGAAGTCGAAGACGATCATATGT
TTTGTGCTGCCATAGTGACGCTATGCAAGTGGAACGTTCTAGAATCAAACTAGGGATGTC
TCTCGACTTTAAACATGCCGCTGTGGCCAGTCCCGACGGCCCGGAACCGACAATGATTGG
TCCGGGAATCATTAAGCACCTAGTTTCCTCCACGTACGGATCATGGATTCTCTTGCCTAA
CGTTTCTGTAACAAACTCCATGAAAGAGAGAAAGAGAAGAAAAAAGAGAGAAATAGATCT
AAAAATATGCAAGAAGTGAAGTAGGGGACTGATATCAGATTAGGTTTTAAAACCCGT

Last Updated on 06.12.2018 16:11