Sequences from confirmation amplicon


Confirmation sequences for line 206H06 and hit At5g51330 are shown.
Line ID 206H06
Locus At5g51330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20861188
Primers used for PCR IS56 - 8474, show primer details
   
Sequence Name 25-K022912-022-206-H06C-8409
GenBank Accession LN497261 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K022912-022-206-H06C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATAGTTCTGAATATAAAAATTG
GGTTTGTTCGCGCATATAAGAGCTGGATCTTATTACGAAATCGATGCTTCGATTCTTCCT
CAGAGATCGCCGGAAAATCTTAAATCGATTAGAGTCGTCATGGTATTCACTCGATTCTCT
GCTTTTTTCACCTTTTATTATAGACAGATCTCGTTTTTTGTTGTTCGTCTGGGTTTTCGA
GTGATTTTTTAAGGTTTATTGATGCAGGTGAGCAAAATCACGGCGAGTGACGTGTCTCTC
CGGTACCCAAGCATGTTTTCACTCCGATCGCATTTCGATTACAGTAGGATGAACCGGAAT
AAACCGATGAAGAAGAGGAGTGGTGGTGGTCTTCTTCCTGTTTTCGACGAGAGTCATGTG
ATGGCTTCGGAGCTAGCTGGAGACTTGCTTTACAGAAGAATCGCACCTCAT
   
Sequence Name 29-K022912-022-206-H06C-IS56
GenBank Accession LN499113 [GenBank]
Sequencing primer gene specific primer, IS56: 5'-ATAGAAAGTTCATGAGGTGCGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K022912-022-206-H06C-IS56
CTGTAAAGCAAGTCTCCAGCTAGCTCCGAAGCCATCACATGACTCTCGTCGAAAACAGGA
AGAAGACCACCACCACTCCTCTTCTTCATCGGTTTATTCCGGTTCATCCTACTGTAATCG
AAATGCGATCGGAGTGAAAACATGCTTGGGTACCGGAGAGACACGTCACTCGCCGTGATT
TTGCTCACCTGCATCAATAAACCTTAAAAAATCACTCGAAAACCCAGACGAACAACAAAA
AACGAGATCTGTCTATAATAAAAGGTGAAAAAAGCAGAGAATCGAGTGAATACCATGACG
ACTCTAATCGATTTAAGATTTTCCGGCGATCTCTGAGGAAGAATCGAAGCATCGATTTCG
TAATAAGATCCAGCTCTTATATGCGCGAACAAACCCAATTTTTATATTCAGAACTATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTC
GTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGT
GTCTAC

Last Updated on 06.12.2018 16:11