Sequences from confirmation amplicon


Confirmation sequences for line 207B08 and hit At3g07430 are shown.
Line ID 207B08
Locus At3g07430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2379375
Primers used for PCR 20tv - 8474, show primer details
   
Sequence Name 57-K117069-0022-207-B08C-20tv
GenBank Accession LN509527 [GenBank]
Sequencing primer gene specific primer, 20tv: 5'-TGATGCATGAATTCTTAAATTTTGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K117069-0022-207-B08C-20tv
TATATAGAATTTAGTTAACAATTATGTATAACAACAAAAACGCTAAAAATCTATATAAAA
GTTACTATTAGTTTAAAAAAAAAAATAGTATTAGTTATTTAAAAGTTATTACTTATTAGT
GTTTATTTACAAATAGTATTAATGATTTAAAAGGATGAAAGATATAATTCTTATTAAAGG
ACGAAAATAATAATTTTGGACAGATTTGGGTTTTATATCTGAAAGACTCAAAAACCTATC
GTCATTTATCTTCAACAGGCGGAATAACGGAGATGGCCGCCATTACAGCTCTCACTCTCC
GCTCTCCTGTTTATCTCCCATCATCTGCCACAAGCCCTAGATTCCATGGCTTCACCAATC
AACCACCACCAGCTCGTCTCTTCTTTCCTCTTAACCCCTTCCCTTCTCTATCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAATT
   
Sequence Name 36-K117068-0022-207-B08C-8409
GenBank Accession LN502377 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K117068-0022-207-B08C-8409
GGGTTAAGAGGAAAGAAGAGACGAGCTGGTGGTGGTTGATTGGTGAAGCCATGGAATCTA
GGGCTTGTGGCAGATGATGGGAGATAAACAGGAGAGCGGAGAGTGAGAGCTGTAATGGCG
GCCATCTCCGTTATTCCGCCTGTTGAAGATAAATGACGATAGGTTTTTGAGTCTTTCAGA
TATAAAACCCAAATCTGTCCAAAATTATTATTTTCGTCCTTTAATAAGAATTATATCTTT
CATCCTTTTAAATCATTAATACTATTTGTAAATAAACACTAATAAGTAATAACTTTTAAA
TAACTAATACTATTTTTTTTTTTAAACTAATAGTAACTTTTATATAGATTTTTAGCATTT
TTGTTGTTATACATAATTGTTAACTAAATTCTATATATACACAAACTAAAATTCTTATTT
TTGAGAAAAATGAAATATTTGGTCAATTTCATGTTTAATATTGTCAAAATTTA

Last Updated on 06.12.2018 16:11