Sequences from confirmation amplicon


Confirmation sequences for line 207D07 and hit At5g08410 are shown.
Line ID 207D07
Locus At5g08410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2710092
Primers used for PCR KT77 - 8474, show primer details
   
Sequence Name 90-K100817-0022-207-D07C-OriL
GenBank Accession LN514150 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K100817-0022-207-D07C-8409
CCGGACTGAGCCATTTACATTGAATATTTCCCGTAATCTTTGATAAAACCTTCCATTCCC
ATCAATTCAACCTCCGGTACACGAACCACATGGTAAACTTTCAACGGAACCGTAACCCTA
ACCCTAGCTCCAATCTTCTCCTTCGCTTTCTCGTCTTCCTCCGTCTCCAACGACGTAGAT
GACGATAGATCAGCGTCCGCGTCTACAGAAGCCGCCGATTTCACAGCAATATCGCAACGA
ATCGTCGATTTTGGATTCTTCCGCGAGATTGAGAAGGCGCATTTGCTTCGACCGTAGGAA
GTAGATNATGAGATAGGTACGAAAATGGACGAAGATGAAGATTGGATCG
   
Sequence Name 90-K100829-0022-207-D07C-KT77
GenBank Accession LN515013 [GenBank]
Sequencing primer gene specific primer, KT77: 5'-AATCGATCAACTCTTCATCTTCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K100829-0022-207-D07C-KT77
GGTCGAGCAATGCGCCTTCTCATCTCGCGGAAGATCCAAAATCGACGATTCGTTGCGATA
TTGCTGTGAAATCGGCGGCTTCTGTAGACGCGGACGCTGATCTATCGTCATCTACGTCGT
TGGAGACGGAGGAAGACGAGAAAGCGAAGGAGAAGATTGGAGCTAGGGTTAGGGTTACGG
TTCCGTTGAAAGTTTACCATGTGGTTCGTGTACCGGAGGTTGAATTGATGGGAATGGAAG
GTTTTATCAAAGATTACGGGAAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTTCAAAATGT
Primers used for PCR KT76 - 8474, show primer details
   
Sequence Name 82-K100817-0022-207-D07C-8409
GenBank Accession LN514150 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K100817-0022-202-D07C-8409
CATTTACATTGAATATATCCATGTAGATTTCCGTGTATACTCATTGCTGTTTATTCCATG
TAGAAAATCTCAGCTAATCTGCCGTTTAAGGTACAGTTCGTGAAAGAGATCGAAGGTCGT
GGTCCTGTCAAATTCTTCACGCATCTTAAGGAAGACGAGTTCGAGTTAATTGATCCGTGA
TTGAATTGAAAAAGGCAAAAACTTTTCTTCTCTTTCTGATATTAGACTTGAAAAATCTTT
TGTGAATGGTTTGTTTGTATGTACTTTTGTATACATTTAGCTTATTATATACATAAGATT
TCACCCATTCTTGAATCCTTTATTGCTGTTTTCTTGCTTCTGATTTGGTTTCCCAGAGTA
TATAGTTCGTCTTGTGTTGGAGAAACTAAAAGCTGTCTATTGATCCATTGACTAGCTNAG
AAGCCTATCANACTGATNTTGCATTTGATGTGTTCACTTCAAATGAACATCATTGAATGA
TCTTATCCTCAAAATCTATCATCCGTTGGAGGTTATGAAGTAGATGGTAGTGAGCATGAA
AGTGGTAAAGATATTGGTTTATGTAACTAAAGAGTGTTTTCAGTTGATTGTTATAGTGAG
AATCTTTGTTTGTAGTTAGAACCAGAAACTAANAGGAATGACATCAGTTGTTTTCTAGGT
TCTTATCCTCTTTGTTTCGTA
   
Sequence Name 82-K100829-0022-207-D07C-KT76
GenBank Accession LN514152 [GenBank]
Sequencing primer gene specific primer, KT76: 5'-TCCCACATTTGATTATACGAAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K100829-0022-202-D07C-KT76
CTGATGTCATTCCTTTTAGTTTCTGGTTCTAACTACAACAAAGATTCTCACTATAACAAT
CAACTGAAAACACTCTTTAGTTACATAAACCAATATCTTTACCACTTTCATGCTCACTAC
CATCTACTTCATAACCTCCAACGGATGATAGATTTTGAGGATAAGATCATTCAATGATGT
TCATTTGAAGTGAACACATCANATGCAAAATCAGTTTGATAGGCTTCTAAGCTAGTCAAT
GGATCAATAGACAGCTTTTAGTTTCTCCAACACAAGACGAACTATATACTCTGGGAAACC
AAATCAGAAGCAAGAAAACAGCAATAAAGGATTCAAGAATGGGTGAAATCTTATGTATAT
NATAAGCTAAATGTATACNAAAGTACATACNAACAAACCATTCACAAAAGATTTTTCAAG
TCTAATATCAGAAAGAGAAGAAAAGTTTTTGCCTTTTTCAATTCAATCACGGATCANTTA
ACTCGAACTCGTCTTCCTTAAGATGCGTGAAGAATTTGACAGGACCACGACCTTCGATCT
CTTTCACGAACTGTACC

Last Updated on 06.12.2018 16:11