Sequences from confirmation amplicon


Confirmation sequences for line 207E07 and hit At2g18740 are shown.
Line ID 207E07
Locus At2g18740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8123706
Primers used for PCR 03vp - 8474, show primer details
   
Sequence Name 62-K117997-0022-207-E07C-03vp
GenBank Accession LN510849 [GenBank]
Sequencing primer gene specific primer, 03vp: 5'-TTCTATTGGCAGGCTCTTATCAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K117997-0022-207-E07C-03vp
CTATCCCTTTTCATTCACAACAAATGCAACAGTTCAAACACAGACTAAATTAGAGAGATA
GTCACACTGCTAATGCATTTCAAACTCTCCTAAAGTTAGCATAACCCTCGAATGTCCTAT
AAACAAAGCCAGGAAATCTCAAGCCATGTAGATTTCATAACCCTGCTAATACGCTTTTTT
CAAGCCATGTAGATTTACAACCTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATAATAAAAGGACNTNT
CGNGGGTTATGCTAACTTTAGGAGAGTTTGAA
   
Sequence Name 22-K117996-0022-207-E07C-8409
GenBank Accession LN496298 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K117996-0022-207-E07C-8409
ATTTACAATTGAATATATCCTGAGGTTGTAAATCTACATGGCTTGAAAAAAGCGTATTAG
CAGGGTTATGAAATCTACATGGCTTGAGATTTCCTGGCTTTGTTTATAGGACATTCGAGG
GTTATGCTAACTTTAGGAGAGTTTGAAATGCATTAGCAGTGTGACTATCTCTCTAATTTA
GTCTGTGTTTGAACTGTTGCATTTGTTGTGAATTGAAAAGGGATAGTTGTCACTGTTACT
ACTACTGCACAAAGAACTGATAAGAGCCTGCCAATAGAA

Last Updated on 06.12.2018 16:11