Sequences from confirmation amplicon


Confirmation sequences for line 207F09 and hit At1g06410 are shown.
Line ID 207F09
Locus At1g06410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1957990
Primers used for PCR 79wk - 3144, show primer details
   
Sequence Name 43-K118466-0022-207-F09C-79wk
GenBank Accession LN505002 [GenBank]
Sequencing primer gene specific primer, 79wk: 5'-TTACATCCCCAACGATAAGAAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K118466-0022-207-F09C-79wk
AAAATCACAATGGACAAGCAACGTTATCTTTACAACATTTTCTTCACAGTTTCGTTTTCG
TTCAGATCCAATAAACAGCCGAATCCAAATTTGATATCCTCAAGAGATCATCCAATTACA
AATAACAATAATAATAGTAATAAGAATATTAAAAAAAAAAGATCAGGAAAAGCTTTTGCT
TGTTGTTAACTTCAACATCCTGCAAACACATTTCCCTGATGAACCCAAACACATCTATTT
TCCTCCTCCTTTGATTGTTTGATTGATTGCTCTGCAAATAACAAAGCTTCTTCACATCAC
CATTTGTTTTTTTCCCCATTATTACTCTTTCACTACCCTTTTCTTCTTTTCTTTCTTTCA
ATCTGACAAACACATACAGACCAACAAACTCCCAGTCTCTTCTATTCCTTGTCTTTGTCT
GTCTAACCTCTTCTTTAGCCATCTATGCACATCAATCATTCATTGTATTTATCAGAACAA
GATCATACATTGTCGCAGAAAGTGAGCAAATATAACTGGAATTGATTGAGATCATAAGAG
GGTAAAACCTGGCAAAAGATCTGGTTCCAAATCAAAGGGCTTCATCAAGTTCACGCATAG
AAAAGTTTGAAGCCTCTGATGCTTCAGCTAGAGATTCAAGCATGCATGTCACTTCAGTAG
TATCGTCCAAGTAGTATTTAGCCTTGCTTGGCTTTTGCCCAACTGTGCATGCAAAGACTA
GAGCATTATCACAGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTAC
CCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAG
TGGGATTGTGCGTCATCCCTTACGTCA
   
Sequence Name 16-K118465-0022-207-F09C-35St
GenBank Accession LN493631 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K118465-0022-207-F09C-35St
CCTTCCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGTACAGGGT
ACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCTGTGATAATGCTCTA
GTCTTTGCATGCACAGTTGGGCAAAAGCCAAGCAAGGCTAAATACTACTTGGACGATACT
ACTGAAGTGACATGCATGCTTGAATCTCTAGCTGAAGCATCAGAGGCTTCAAACTTTTCT
ATGCGTGAACTTGATGAAGCCCTTTGATTTGGAACCAGATCTTTTGCCAGGTTTTACCCT
CTTATGATCTCAATCAATTCCAGTTATATTTGCTCACTTTCTGCGACAATGTATGATCTT
GTTCTGATAAATACAATGAATGATTGATGTGCATAGATGGCTAAAGAAGAGGTTAGACAG
ACAAAGACAAGGAATAGAAGAGACTGGGAGTTTGTTGGTCTGTATGTGTTTGTCAGATTG
AAAGAAAGAAAAGAAGAAAAGGGTAGTGAAAGAGTAATAATGGGGAAAAAAACAAATGGT
GATGTGAAGAAGCTTTGTTATTTGCAGAGCAATCAATCAAACAATCAAAGGAGGAGGAAA
ATAGATGTGTTTGGGTTCATCAGGGAAATGTGTTTGCAGGATGTTGAAGTTAACAACAAG
CAAAAGCTTTTCCTGATCTTTTTTTTTTAATATTCTTATTACTATTATTATTGTTATTTG
TAATTGGATGATCTCTTGAGGATATCAAATTTGGATTCGGCTGTTTA
Primers used for PCR 50wi - 8474, show primer details
   
Sequence Name 42-K118466-0022-207-F09C-50wi
GenBank Accession LN504638 [GenBank]
Sequencing primer gene specific primer, 50wi: 5'-GTGTCTTTTTCTTAGGTGGTCAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K118466-0022-207-F09C-50wi
AGATCGTGGAACCTGTAATGAAGCAGTACACTGAATCGACTGATGGCTCTTCCATTGAAA
TCAAAGAGAGCGCTTTGGTTTGGCAATATCGGGATGCAGATCCAGGCTTTGGTTCTTTAC
AAGCGAAGGAAATGCTAGAGCATTTAGAGAGTGTTCTAGCTAATGAGCCAGTGGCAGTCA
AAAGCGGTCACTACATAGTAGAAGTCAAGCCTCAGGTAAGTTACCTCTCTTCTGCTCAAT
TTGAGCATTAAGGTTGTCGAAGATATCTAATTATAGACGTTTGTGCTTGTTCTTGTGTGA
GAATAGGGAGTGAGCAAAGGATCCGTGTCAGAAAAGATATTTTCATCAATGGCCGGAAAG
GGAAAACCGGTTGATTTCGTGTTATGTATTGGAGATGACAGATCTGATGAAGACATGTTT
GAAGCGATTGGTAATGCGATGTCGATAGTTCAATTACATCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 15-K118465-0022-207-F09C-8409
GenBank Accession LN493184 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K118465-0022-207-F09C-8409
TTGATGTAATTGAACTATCGACATCGCATTACCAATCGCTTCAACATGTCTTCATCAGAT
CTGTCATCTCCAATACATAACACGAAATCAACCGGTTTTCCCTTTCCGGCCATTGATGAA
AATATCTTTTCTGACACGGATCCTTTGCTCACTCCCTATTCTCACACAAGAACAAGCACA
AACGTCTATAATTAGATATCTTCGACAACCTTAATGCTCAAATTGAGCAGAAGAGAGGTA
ACTTACCTGAGGCTTGACTTCTACTATGTAGTGACCGCTTTTGACTGCCACTGGCTCATT
AGCTAGAACACTCTCTAAATGCTCTAGCATTTCCTTCGCTTGTAAAGAACCAAAGCCTGG
ATCTGCATCCCGATATTGCCAAACCAAAGCGCTCTCTTTGATTTCAATGGAAGAGCCATC
AGTCGATTCAGTGTACTGCTTCATTACAGGTTCCACGATCTGCATCCACCCAAAATCAGA
GCTCTGCCCACATGTCTCCCATTCTTCACTGCCTGACCACCTAAAAAAAAAGACAC

Last Updated on 06.12.2018 16:11