Sequences from confirmation amplicon


Confirmation sequences for line 207G11 and hit At1g74680 are shown.
Line ID 207G11
Locus At1g74680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28060797
Primers used for PCR 21tv - 8474, show primer details
   
Sequence Name 43-K117029-0022-207-G11C-21tv
GenBank Accession LN504976 [GenBank]
Sequencing primer gene specific primer, 21tv: 5'-ACCTTGAGTTGAAGGATTTGACTG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K117029-0022-207-G11C-21tv
AGCATAAAGAGAATGTAGTATATTTCACATACATGATCTTAACTCGAACCGTTTCTATCA
AACATTTCAGATCTACGGTAGCGATTCTTTCTATGAACATCAAACTGAAGTGAAGAAAGC
TTATGCGAAACCGCTGACCAAATCATATTCACAGAATCTCCAACTTGCGAAGGAAAGCGA
TACTCAAAACACCCAGCAACCTCTTTCAATCTCCCCCACTTTTTCTCCATCATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGGTTATTAT
   
Sequence Name 11-K117028-0022-207-G11C-8409
GenBank Accession LN491292 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K117028-0022-207-G11C-8409
GAGAAAAGTGGGGGAGATTGAAAGAGGTTGCTGGGTGTTTTGAGTATCGCTTTCCTTCGC
AAGTTGGAGATTCTGTGAATATGATTTGGTCAGCGGTTTCGCATAAGCTTTCTTCACTTC
AGTTTGATGTTCATAGAAAGAATCGCTACCGTAGATCTGAAATGTTTGATAGAAACGGTT
CGAGTTAAGATCATGTATGTGAAATATACTACATTCTCTTTATGCTTCATGTCTGTTTTC
TTTTCTTGAGTTGAAAAAGATAACAGTCAAATCCTTCAACTCAAGGT

Last Updated on 06.12.2018 16:11