Sequences from confirmation amplicon


Confirmation sequences for line 208E09 and hit At2g39010 are shown.
Line ID 208E09
Locus At2g39010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16293298
Primers used for PCR AS14 - 8474, show primer details
   
Sequence Name 03-K022912-022-208-E09C-8409
GenBank Accession LN487303 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K022912-022-208-E09C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTAAGTAATCTTGGTACCTATGATTCATTC
AAATTTAAATGTCTATGTCATTGATTGTTAATGACGTAAGTTGATTTATTTTGTATGAAA
TTATTGAATTGTAGGTATTAGCACCATTGCCAATTGGATTTTCCGTGGTCATGGTTCATT
TAGCCACAATTCCAATTACTGGCACCGGAATAAACCCGGCTAGGAGTTTTGGAGCTGCCG
TCATCTACAACAATCAAAAGGCTTGGGATGATCAGGTTTGTATTTTCACCATCTGATATT
CGTTCTTGGGTTTTAAATTAACATATTAATGAGTTTTGTTTTAATAACTACAGTGGGATC
TTCTGGGTCGGTCCATTTGTGGGTGCAGCCATTGCAGCATTTTA
   
Sequence Name 07-K022912-022-208-E09C-AS14
GenBank Accession LN489077 [GenBank]
Sequencing primer gene specific primer, AS14: 5'-GCTCTCAACACAAACTGATGGTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K022912-022-208-E09C-AS14
GCTGCAATGGCTGCACCCACAAATGGACCGACCCAGAAGATCCACTGTAGTTATTAAAAC
AAAACTCATTAATATGTTAATTTAAAACCCAAGAACGAATATCAGATGGTGAAAATACAA
ACCTGATCATCCCAAGCCTTTTGATTGTTGTAGATGACGGCAGCTCCAAAACTCCTAGCC
GGGTTTATTCCGGTGCCAGTAATTGGAATTGTGGCTAAATGAACCATGAACACGGAAAAT
CCAATTGGCAATGGTGCTAATACCTACAATTCAATAATTTCATACAAAATAAATCAACT

Last Updated on 06.12.2018 16:11