Sequences from confirmation amplicon


Confirmation sequences for line 208H02 and hit At4g08850 are shown.
Line ID 208H02
Locus At4g08850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:5638437
Primers used for PCR JD12 - 8474, show primer details
   
Sequence Name 32-K102590-0022-208-H02C-8409
GenBank Accession LN500534 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K102590-0022-208-H02C-8409
TATCCTTGGATAAAAATTGGTGCGTTTCGAAACGCTGCGTTATCTGGAATTGGACCTTGG
AGGTTGTTGTGTGATACATCGACGTGTGTTAGTGCTAGCATATCTTTGAAACTTGGTGGG
ATTTGACCTGAGAGATTGTTGTGTGAGAGGTCGAGTCTTTCGAGGTTTTGTAAAGATCTG
AATTGTGATGAGATCTCTCCGTCGAGCTGATTGTAGCTGAGATCAAGCATTTGTAACTGA
GATAGCTTTGTTAACCCCTCCGGGATGGTTTGATCCAAGTCGTTTCTGCTCAGGTTCATG
TAGTAGAGCCTTGGCAAATTGTTGAGCGTTGGGGGTATTTCGGAACTGAATCGGTTTGAG
GATAGATCAAGATACTCAAGATTGGTTAAGAGTCTGATTCCTGAAGGGATTTTGCCTGAT
AGCCGATTCCCGTTGAGTTGGAGTTTTGATATACGGTTGATATTGCTGATTGATTCAGGT
AGCTCGCCGGTGATGCGGTTGGAAGATAGATCGAGCTGACTTAGCTGTGTC
   
Sequence Name 32-K102591-0022-208-H02C-JD12
GenBank Accession LN500535 [GenBank]
Sequencing primer gene specific primer, JD12: 5'-GAGATTTGGAACATGACACAGCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K102591-0022-208-H02C-JD12
CTTCACGCATCACCGGCGAGCTACCTGAATCATCAGCAATATCAACCGTATATCAAAACT
CCAACTCAACGGGAATCGGCTATCAGGCAAAATCCCTTCAGGAATCAGACTCTTAACCAA
TCTTGAGTATCTTGATCTATCCTCAAACCGATTCAGTTCCGAAATACCCCCAACGCTCAA
CAATTTGCCAAGGCTCTACTACATGAACCTGAGCAGAAACGACTTGGATCAAACCATCCC
GGAGGGGTTAACAAAGCTATCTCAGTTACAAATGCTTGATCTCAGCTACAATCAGCTCGA
CGGAGAGATCTCATCACAATTCAGATCTTTACAAAACCTCGAAAGACTCGACCTCTCACA
CAACAATCTCTCAGGTCAAATCCCACCAAGTTTCAAAGATATGCTAGCACTAACACACGT
CGATGTATCACACAACAACCTCCAAGGTCCAATTCCAGATAACGCAGCGTTTCGAAACGC
ACCAATTTTTATCCAAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGAT
AAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAA
TTCGGAAATCTTTATTTCGACGTGTCTACATT
Primers used for PCR 35wp - 8474, show primer details
   
Sequence Name 82-K118629-0022-208-H02C-35wp
GenBank Accession LN514242 [GenBank]
Sequencing primer gene specific primer, 35wp: 5'-TATAAGACAAAGCATGAGCCACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K118629-0022-208-H02C-35wp
TCCATCTAACTTCTTCGCTTCTCATCATTCTCCAAACTTTCCTCAAGCTTCCTCTCTCCA
TGTACTCGTAAACCAGAAACGTGTTGCGGCGATGCGAGCAGAATCCGAAGAGTTTCACGA
CGTTCCGGTGACGGATCTCGGTTAGTGCTCTGATTTCGTTGAGAAACTCTTGTTTTGTTG
ATGGATTTGAGATTGATGAGTCTGTTGTTTCGTTTAGCTTTTTCACCGCCATGATTGCGT
TTGGTAGCTTTGCTTTGTAGACTTTGCCGTGTCCTCCGGTTCCGATTAGGTATTTTGGAT
CGAATTCTCCTGTTGCTTTGATGATCTCTTGGTATCGGACTTTGCCGTCGAAGCTGAAGA
TTGATAATGTTTCTCCTCCTGATTCTGAATCTGTATGCTCCTCGATTTGTTTTGTTCGTT
TACGGAAACAGATGAAGATTCCGGCGCAGACGGAGAGGATTATGATTGCTCCGATGATCG
GAACTAGTATGTAGATGATTAGGTTTCGATCCTTGTGTGATTTCTTTGAGGATGTGATTG
AACATGGCTTCAAGCCTTGTGTGGTGTTAACACTACCACATAAGTCTTTGTTACCTTCAA
AAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGC
   
Sequence Name 63-K118628-0022-208-H02C-oriL
GenBank Accession LN511089 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K118628-0022-208-H02C-oriL
TACACCACAAAGGCTTGAAGCCATGTTCATCACATCCTCAAAGAAATCACACAAGGATCG
AAACCTAATCATCTACATACTAGTTCCGATCATCGGAGCAATCATAATCCTCTCCGTCTG
CGCCGGAATCTTCATCTGTTTCCGTAAACGAACAAAACAAATCGAGGAGCATACAGATTC
AGAATCAGGAGGAGAAACATTATCAATCTTCAGCTTCGACGGCAAAGTCCGATACCAAGA
GATCATCAAAGCAACAGGAGAATTCGATCCAAAATACCTAATCGGAACCGGAGGACACGG
CAAAGTCTACAAAGCAAAGCTACCAAACGCAATCATGGCGGTGAAAAAGCTAAACGAAAC
AACAGACTCATCAATCTCAAATCCATCAACAAAACAAGAGTTTCTCAACGAAATCAGAGC
ACTAACCGAGATCCGTCACCGGAACGTCGTGAAACTCTTCGGATTCTGCTCGCATCGCCG
CAACACGTTTCTGGTTTACGAGTACATGGAGAGAGGAAGCTTGAGGAAAGTTTTGGAGAA
TGATGATGAAGCGAAGAAGTTAGATTGGGGAAAGAGGATCAACGTTGTGAAAGGTGTGGC
TCATGCTTGGTCTTAT
Primers used for PCR MN50 - 8474, show primer details
   
Sequence Name 81-K118629-0022-208-H02C-MN50
GenBank Accession LN514120 [GenBank]
Sequencing primer gene specific primer, MN50: 5'-ATTCTCCTGTTGCTTTGATGATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K118629-0022-208-H02C-MN50
GATTGATAATGTTTCTCCTCCTGATTCTGAATCTGTATGCTCCTCGATTTGTTTTGTTCG
TTTACGGAAACAGATGAAGATTCCGGCGCAGACGGAGAGGATTATGATTGCTCCGATGAT
CGGAACTAGTATGTAGATGATTAGGTTTCGATCCTTGTGTGATTTCTTTGAGGATGTGAT
TGAACATGGCTTCAAGCCTTGTGTGGTGTTAACACTACCACATAAGTCTTTGTTACCTTC
AAAAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCACACAGTTATT
   
Sequence Name 62-K118628-0022-208-H02C-8409
GenBank Accession LN510859 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K118628-0022-208-H02C-8409
TACACCACACAAGGCTTGAAGCCATNTTCAATCACATCCTCAAAGAAATCACACAAGGAT
CGAAACCTAATCATCTACATACTAGTTCCGATCATCGGAGCAATCATAATCCTCTCCGTC
TGCGCCGGAATCTTCATCTGTTTCCGTAAACGAACAAAACAAATCGAGGAGCATACAGAT
TCAGAATCAGGAGGAGAAACATTATCAATCTTCAGCTTCGACGGCAAAGTCCGATACCAA
GAGATCATCAAAGCAACAGGAGAAT

Last Updated on 06.12.2018 16:11