Sequences from confirmation amplicon


Confirmation sequences for line 209A05 and hit At4g13500 are shown.
Line ID 209A05
Locus At4g13500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7857268
Primers used for PCR 35ra - 8474, show primer details
   
Sequence Name 84-K115895-0022-209-A05C-35ra
GenBank Accession LN514468 [GenBank]
Sequencing primer gene specific primer, 35ra: 5'-GGCTAGTGATTCCTCAACTTCATC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K115895-0022-209-A05C-35ra
TACTCATTCTTTTGTCCCCATCACCATTAGGATTCCCAGAATTCAAGCTTCATGCCAAAT
TAGGTACTCTTATATCTTTTATTTGTTTGTAGATGTAAATTTTGTTAATCTTTGGTTATT
GCATAAGGTGGAGGAGATGGAGAAGTGAAGCCTAAAGATAAGAAAAAGTTTATAACCAAA
GAGGAAGAACCTGAACAGTAAGTTAATCTTAATTCTCAATTTTCATCATGAGATTGTAGA
AAATAACAGAAACTCATAGAATGGTAATGTAATTTTGCAAAATCACAGAAAAACAAACCT
AGGAAATTGATGAAAACATGGAAAATTACAAAACGATATGTAGGTTACCAATTTAATACA
AAAATTTTAGTCTCCATTGCAGAATTTAAGTAGTTTTTTTTTTTGTATGTGTCAATGACA
TGTAAACGGGGGGTTTGTGACATTGTGTTAGTATTGTATCAATATCCAAACTCAAATCCG
TGTTAGCAAAAAAATAAAAATATTTGGAAATAAACTCTACATGGCTATTTTCTCACATGA
AATAAAATATTCACCGAGAAACATCCGGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACTTGGATCACCATTGAGTATGATGGTCAATATGGAGAAAAAAAAAGAGTAT
TTACCATTTTTTTTTCATTTCAAAAATGAAAATTTCC
   
Sequence Name 54-K115894-0022-209-A05C-8409
GenBank Accession LN508587 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K115894-0022-209-A05C-8409
ATTTCATTGAATATATCCTGGATGTTCCTCGGTGAATATTTTATTTCATGTGAGAATATA
GCCATGTAGAGTTTATTTCCAAATATTTTTATTCTTTTGCTAACACGGATTTGAGTCTGG
ATATTGATACAATACTAACACAATGTCACAAACCACCAGTCTACATGTCATTGACACATA
CAAAAAAAAAAACTACTTAAATTCTGCAAGGGAAACAAAAATTTTTGTATTAAATTGGTA
ACCTACATATCGTTTTGTAATTTTCCATGTTTTCATCAATTTCCAAGGTTTGTTTTTCTG
TGATTTTGCAAAATTACATTACCATTCTATGATTTTCTGTTATTTTCAACAATCTCAGGA
TAAAAATTGAAAATTAAAATTAACTTACGGTTCAGGTTCTTCCTCTTTGGTTATAAACTT
TTTCTTATCTTTAGGCTTCACTTCTCCATCTCCTCCACCTTAGGCAATACCCAAAAATTA
ACAAATTTTACTTCTCCAAACAAATAAAAAATATAAAAGTACCTAATTTGGCATGAACCT
GGAATTCTGGAAATCCAAAGGGTGATGGTGGACAAAAAAATTGAGGTAAAGCAGGCATTG
ATAAATATAAAAAAAATAATAAATTTAA

Last Updated on 06.12.2018 16:11