Sequences from confirmation amplicon


Confirmation sequences for line 209A08 and hit At1g16380 are shown.
Line ID 209A08
Locus At1g16380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5599008
Primers used for PCR UK04 - 8474, show primer details
   
Sequence Name 52-K018511-022-209-A08C-UK04
GenBank Accession LN507672 [GenBank]
Sequencing primer gene specific primer, UK04: 5'-CTTCGTGTATCGATCGTGTTTCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K018511-022-209-A08C-UK04
TTCATAAACACCAGAGGATCGACGGGAAAACCACGAATGACGGGGAAGTTTTTCGACATA
TGAACCGGAAGGTTCTAAAGCAAGCTCAATGTTCGATTGGTATCTTTGTGGATAGAAACA
TAACAGGGTTTCACCAGCTTCACGGGTCTGATTCGGTACAACACGTTGCTGCATTGTTCT
TCGGTGGTCCTGATGATCGTGAGGCTCTGTCCTTGTGCAAGTGGCTTACCAATAACTCTC
AGATTCACCTCACTGTCATACAGTTTGTGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTACATTCACGT
   
Sequence Name 53-K018511-022-209-A08C-8409
GenBank Accession LN507997 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K018511-022-209-A08C-8409
CATTTCCCGGACATGAAGCCACAAACTGTATGACAGTGAGGTGAATCTGAGAGTTATTGG
TAAGCCACTTGCACAAGGACAGAGCCTCACGATCATCAGGACCACCGAAGAACAATGCAG
CAACGTGTTGTACCGAATCAGACCCGTGAAGCTGGTGAAACCCTGTTATGTTTCTATCCA
CAAAGATACCAATCGAACATTGAGCTTGCTTTAGAACCTTCCGGTTCATATGTCGAAAAA
CTTCCCCGTCATTCGTGGTTTTCCCGTCGATCCTCTGGTGTTTATGGAAAGGTAGAAACA
CGATCGATACACGAA

Last Updated on 06.12.2018 16:11