Sequences from confirmation amplicon


Confirmation sequences for line 209F12 and hit At4g18910 are shown.
Line ID 209F12
Locus At4g18910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10366651
Primers used for PCR AW02 - 8474, show primer details
   
Sequence Name 56-K018604-022-209-F12C-AW02
GenBank Accession LN508969 [GenBank]
Sequencing primer gene specific primer, AW02: 5'-AGAGAAATACAGTTATGGCGGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K018604-022-209-F12C-AW02
GATGCTAGAGACGGAGCTGTGGTGGTCAATCTCAAGGAAGAAGACGAACAACAACAACAA
CAACAAGCTATTCATAAACCCTTGAAGAAACAAGACTCTCTCCTCTCTATCTCTGTCCCT
TTCTTACAAAAGGTTTTTTACTTTTGTCTTTACTCCTGTAACTCTCAATCTCCAAAACCT
CTTTAGTTTTGAAGAAGAAAAAATATGTCTTACAAATCTTTAGTTTTGCTCTGTTTTTTA
AATTGTCAAATTGCTCTGTTTTCAGTTGATGGCGGAGGTTTTGGGAACATACTTCTTGAT
ATTCGCCGGTTGTGCCGCGGTGGCTGTAAACACACAACATGACAAAGCCGTGACTCTTCC
AGGGATCGCCATCGTTTGGGGACTTACCGTCATGGTCCTTGTTTACTCTCTCGGATATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGTATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTC
GTATTTATAGGCGAAAGCAATAAACAAATTATTTCTAATTCGGAAATCTTTATTTCGACG
TGTCTACATTC
   
Sequence Name 57-K018604-022-209-F12C-8409
GenBank Accession LN509272 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K018604-022-209-F12C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCGAGAGAGTAAACAAGGACC
ATGACGGTAAGTCCCCAAACGATGGCGATCCCTGGAAGAGTCACGGCTTTGTCATGTTGT
GTGTTTACAGCCACCGCGGCACAACCGGCGAATATCAAGAAGTATGTTCCCAAAACCTCC
GCCATCAACTGAAAACAGAGCAATTTGACAATTTAAAAAACAGAGCAAAACTAAAGATTT
GTAAGACATATTTTTTCTTCTTCAAAACTAAAGAGGTTTTGGAGATTGAGAGTTACAGGA
GTAAAGACAAAAGTAAAAAACCTTTTGTAAGAAAGGGACAGAGATAGAGAGGAGAGAGTC
TTGTTTCTTCAAGGGTTTATGAATAGCTTGTTGTTGTTGTTGTTGTTCGTCTTCTTCCTT
GAGATTGACCACCACAGCTCCGTCTCTAGCATCACCACCGTTTCCCGAGATCTCCGCCAT
AAC

Last Updated on 06.12.2018 16:11