Sequences from confirmation amplicon


Confirmation sequences for line 210A10 and hit At5g20550 are shown.
Line ID 210A10
Locus At5g20550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6953522
Primers used for PCR CN68 - 8474, show primer details
   
Sequence Name 45-K104270-0022-210-A10C-CN68
GenBank Accession LN505520 [GenBank]
Sequencing primer gene specific primer, CN68: 5'-TAGCCATCGCTTTAAAGACTTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K104270-0022-210-A10C-CN68
GTTTCCCTGAAAAACTGACTGTCAAAATCACCTAAGTACATGCTATTGAAACTGAAAGGT
ATAATAAGCTTTACCTGAATCCGACAGGGACATCAGGCCAGAACTTAAGTTGTCTCTGAT
CTTCAGGGTAAGTAGTGATATACAAACGGTCGATCCAATCAAGAACTTGATCATCCCACA
GAATCATATCGTTACCATATCCTTGGATACTACCAATCTCTCTCGCGTACTTTTGTTTCT
CTTCGGACGGAAGTGCACAGAACTCCTTGGTTAGCTTGTAAATCTTGTCGAGCAACGCTT
TTGTAATTCCATGATTTATAAATTCATGTCCGGGAAATCTACATGAATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGTTATT
   
Sequence Name 61-K104271-0022-210-A10C-8409
GenBank Accession LN510511 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K104271-0022-210-A10C-8409
GGAATTACAAAAGCGTTGCTCGACAGATTTACAAGCTAACCAAGGAGTTCTGTGCACTTC
CGTCCGAAGAGAAACAAAAGTACGCGAGAGAGATTGGTAGTATCCAAGGATATGGTAACG
ATATGATTCTGTGGGATGATCAAGTTCTTGATTGGATCGACCGTTTGTATATCACTACTT
ACCCTGAAGATCAGAGACAACTTAAGTTCTGGCCTGATGTCCCTGTCGGATTCAGGTAAA
GCTTATTATACCTTTCAGTTTCAATAGCATCTACTTAGGTGATTTTGACAGTCAGTTTAT
TCAGGGAAACTTTACATGAATACACAATGAAGCAACATCTAGTGTTTAATCAAGTCTTTA
AAGCGATGGC

Last Updated on 06.12.2018 16:11