Sequences from confirmation amplicon


Confirmation sequences for line 210E12 and hit At5g24510 are shown.
Line ID 210E12
Locus At5g24510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8369560
Primers used for PCR KT73 - 8474, show primer details
   
Sequence Name 50-K100815-0022-210-E12C-8409
GenBank Accession LN507093 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K100815-0022-210-E12C-8409
ATTTACATTGAATATATCCTGTGAGAAGAAGAACATCGACGATCTCATCATGAATGTAGG
AGCCGGTGGTTGTGGTGTGGCTCGTCCAGTTACCACTGCTGCTCCTACCGCTTCTCAATC
AGTCTCTATTCCCGAGGAGAAGAAGAATGAGATGGTATTTATTATCACATTATTAATTAC
AACAAACTACTAAATCAAATACTAAATGGTATAATTGTTTTATAATCTTAGGAGGTGATC
AAAGAAGAGAGTGAAGATGACATGATAATCGGATTATTTGACTAGAAGAGCTTCTTTTTC
TTTGCTTCCAAATTTTATGAATTTTGCCTCATATGAATATTTTGATTATTTACAATTTAT
TTGATTGTTTATGAGAGACAACAAAAATAAATCAAATCTATTATTGGCTATTGTTTAATA
AATATATACAACAACAAAAATATGGGGGA
   
Sequence Name 50-K100827-0022-210-E12C-KT73
GenBank Accession LN507095 [GenBank]
Sequencing primer gene specific primer, KT73: 5'-TCCCCCATATTTTTGTTGTTGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K100827-0022-210-E12C-KT73
CATATAGATTTGATTATTTTTGTTGTCTCTCATAAACATCAAATAAATTGTAAATAATCA
AAATATTCATATGAGGCAAAATTCATAAAATTTGGAAGCAAAGAAAAAGAAGCTCTTCTA
GTCAAATAATCCGATTATCATGTCATCTTCACTCTCTTCTTTGATCACCTCCTAAGATTA
TAAAACAATTATACCATTTAGTATTTGATTTAGTAGTTTGTTGTAATTAATAATGTGATA
ATAAATACCATCTCATTCTTCTTCTCCTCGGGAATAGAGACTGATTGAGAAGCGGTAGGA
GCAGCAGTGGTAACTGGACGAGCCACACCACAACCACCGGCTCCTACATTCATGATGAGA
TCGTCGATGTTCTTCTTCTCACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11