Sequences from confirmation amplicon


Confirmation sequences for line 210H07 and hit At1g10850 are shown.
Line ID 210H07
Locus At1g10850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3613304
Primers used for PCR 13sa - 8474, show primer details
   
Sequence Name 48-K116229-0022-210-H07C-13sa
GenBank Accession LN506610 [GenBank]
Sequencing primer gene specific primer, 13sa: 5'-AATAAGCTCTCTGGTCAAATTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K116229-0022-210-H07C-13sa
AAACCAACGCCTATACCTAAGTCTAAGAAGAGCAAAGCGAAGCTGATCGGGATTATCGCC
GGAAGCGTTGCCGGCGGAGTACTAGTCCTTATCTTGCTATTGACGTTGCTTATCGTCTGC
TGGCGACGGAAGCGGCGGAACCAGGCACCCGAGGGAAGATAGAAAAGGTAAAGGAATAGC
GGAAGCAGAAGGAGCAACAACTGCAGAGACAGAGAGAGACATTGAGAGGAAAGACAGAGG
ATTCTCATGGGAAAGAGGCGAAGAAGGTGCAGTGGGAACTCTGGTTTTCCTTGGAACCAG
TGATTCAGGCGAGACGGTAGTGAGATACACCATGGAAGATCTGCTAAAAGCTTCGGCAGA
GACATTGGGAAGAGGAACGTTGGGGAGCACGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATT
   
Sequence Name 08-K116228-0022-210-H07C-8409
GenBank Accession LN489883 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K116228-0022-210-H07C-8409
CGAAGCTTTTAGCAGATCTTCCATGGTGTATCTCACTACCGTCTCGCCTGAATCACTGGT
TCCAAGGAAAACCAGAGTTCCCACTGCACCTTCTTCGCCTCTTTCCCATGAGAATCCTCT
GTCTTTCCTCTCAATGTCTCTCTCTGTCTCTGCAGTTGTTGCTCCTTCTGCTTCCGCTAT
TCCTTTACCTTTTTCTATCTTCCCTCGGTGCCTGGTTCCGCCGCTTCCGTCGCCAGCAGA
CGATAAGCAACGTCAATAGCAAGATAAGGACTAGTACTCCGCCGGCAACGCTTCCGGCGA
TAATCCCGATCAGCTTCGCTTTGCTCTTCTTAGACTTAGGTATAGGCGTTGGTTTCGCAG
ACGGCGCAGGTGAGATTCCGCATGGACTTCCAATCTGATCGCCGCAAAGAGCGACGTTTC
CGGTAAACGATGACTCATCAAACTGCTTCAAAGCTCGTGTCAGTGGAATTTGACCA

Last Updated on 06.12.2018 16:11