Sequences from confirmation amplicon


Confirmation sequences for line 211D07 and hit At4g25360 are shown.
Line ID 211D07
Locus At4g25360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12970009
Primers used for PCR BL95 - 8474, show primer details
   
Sequence Name 29-K105305-0022-211-D07C-BL95
GenBank Accession LN499285 [GenBank]
Sequencing primer gene specific primer, BL95: 5'-CCTGATTGAACATCAGTCTTTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K105305-0022-211-D07C-BL95
ATCCTCTTTTCTTGTGTCAAGGTGTCTCAACTTCCTATCACTACTGAAATCTGGAGGTGG
TATACTTTTGCTAACAACAACAGGTAAGCCAGAGCTAGAATCAGAAGTCAGATTTTTATC
AAACGCATTGATGTCACTACTCTTCTGAAGGATATTGACAGTATGGTTGCTCAAAGATAA
AGGGACATGAACTTGTTCAGGATTCCCTATCCCATAAAATATGCCACCAACTGAAGAACT
ATTCGGATACGAAAGACGAAGCAACAATCCGAAAACAAAGAATGATGCCAAACCACCAAT
AGCAATTGCAACTGTAGACACTTTCCGAGGAAATCCAGCCGCAGCTAATATAGAATTATT
CCTAGGAGAACCAAAAGCCATGGTTTCTTCACTAGTAGATACCCAGAACCAATACAAGCA
ACAAGAACCAGAGCTTATAGGTGATAATTCAATATCAACATTATTCAAAATGTTATCTTC
AGGGGCATGCAATACGCAAGTGAACTAAGTGAATCTGTTACTTGATTCAATCTTCCACTG
GATCACAGATAAGATTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATTCC
   
Sequence Name 13-K105306-0022-211-D07C-8409
GenBank Accession LN492035 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K105306-0022-211-D07C-8409
CATTTACATTGAAATCTTATCTGTGATCCAGTGGAAGATTGAATCAAGTAACAGATTCAC
TTAGTTCACTTGCGTATTGCATGCCCCTGAAGATAACATTTTGAATAATGTTGATATTGA
ATTATCACCTATAAGCTCTGGTTCTTGTTGCTTGTATTGGTTCTGGGTATCTACTAGTGA
AGAAACCATGGCTTTTGGTTCTCCTAGGAATAATTCTATATTAGCTGCGGCTGGATTTCC
TCGGAAAGTGTCTACAGTTGCAATTGCTATTGGTGGTTTGGCATCATTCTTTGTTTTCGG
ATTGTTGCTTCGTCTTTCGTATCCGAATAGTTCTTCAGTTGGTGGCATATTTTATGGGAT
AGGGAATCCTGAACAAGTTCATGTCCCTTTATCTTTGAGCAACCATACTGTCAATATCCT
TCAGAAGAGTAGTGACATCAATGCGTTTGATAAAAATCTGACTTCTGATTCTAGCTCTGG
CTTACCTGTTGTTGTTAGCAAAAGTATACCACCTCCAGATTTCAGTAGTGATAGGAAGTT
GGAGACACCTTTGACACAAGAAAAAGAGGATTTGGTTTCTTCGGATATAACTGAAAAGAC
TGAT

Last Updated on 06.12.2018 16:11