Sequences from confirmation amplicon


Confirmation sequences for line 211D12 and hit At1g64440 are shown.
Line ID 211D12
Locus At1g64440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23937747
Primers used for PCR MP03 - 8474, show primer details
   
Sequence Name 28-K017191-022-211-D12C-MP03
GenBank Accession LN498632 [GenBank]
Sequencing primer gene specific primer, MP03: 5'-CTCAGCATTATGATTCTCCATTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K017191-022-211-D12C-MP03
ATTTCCCCGGATCCCACGTTGTACATCACGGCAAATGTCCTCTATGAACAGCTACGAAAT
GAGAAACATTCCAGTATAAATACCAATCAAATCTACAATAAAAGCTTCCACTTTGCTATC
GAAAACGACTGAGTACCTTTGTCCGTCCATAAGGACTCATTCCAGACAGGGGAGACTCTT
CTGTACAAGGAACCTCCTTTGGCCAGCCATACACAGTAGCGGACGAAGAAAATACAAGCT
GATTGAGATCAATGAGAGAAAGTTAAATCATGTTTCACTGTCACATACTCACATACATAA
GGAATACCAAAAATCTGCAATACAGACCAAAAGGGGATTTTACCTTTTTACATCCGTGTG
CAGCCATTACTTCCAAAAGTGTAATAGTCGCAATCAAGTTATTGTTATAATACAGAAGTG
GTTTCGCCACGCTCTCACCAACTGCTTTCAATCCAGCAAAATGCATTACTGCATCAAACC
TAGATTACAGCCACATAGAGCACTAAATCACATCTCTGGAGGACTGGAAACAAGAAGCAT
TTCACTGTAGATTCAGATCTTAGCACGAAACATACAAAACATACAAAACATACAAAGCAG
GATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
   
Sequence Name 32-K017191-022-211-D12C-8409
GenBank Accession LN500370 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K017191-022-211-D12C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCTTTGTATGTTT
TGTATGTTTTGTATGTTTCGTGCTAAGATCTGAATCTACAGTGAAATGCTTCTTGTTTCC
AGTCCTCCAGAGATGTGATTTAGTGCTCTATGTGGCTGTAATCTAGGTTTGATGCAGTAA
TGCATTTTGCTGGATTGAAAGCAGTTGGTGAGAGCGTGGCGAAACCACTTCTGTATTATA
ACAATAACTTGATTGCGACTATTACACTTTTGGAAGTAATGGCTGCACACGGATGTAAAA
AGGTAAAATCCCCTTTTGGTCTGTATTGCAGATTTTTGGTATTCCTTATGTATGTGAGTA
TGTGACAGTGAAACATGATTTAACTTTCTCTCATTGATCTCAATCAGCTTGTATTTTCTT
CGTCCGCTACTGTGTATGGCTGGCCAAAGGAGGTTCCTTGTACAGAAGAGTCTCCCCTGT
CTGGAATGAGTCCTTATGGACGGACAAAGGTACTCAGTCGTTTTCGATAGCAAAGTGGAA
GCTTTTATTGTAGATTTGATTGGTATTTATACTGGAATGTTTCTCATTTCGTAGCTGTTC
ATAGAGGACATTTGCCGTGATGTACAACGTGGTGATCCTGAATGGA

Last Updated on 06.12.2018 16:11