Sequences from confirmation amplicon


Confirmation sequences for line 211E03 and hit At2g27880 are shown.
Line ID 211E03
Locus At2g27880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11874640
Primers used for PCR PH25 - 8474, show primer details
   
Sequence Name 01-K017512-022-211-E03C-PH25
GenBank Accession LN486373 [GenBank]
Sequencing primer gene specific primer, PH25: 5'-AATGATGATCTGAGCAAGGAGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K017512-022-211-E03C-PH25
GTCAGTGACTACCCAACTAGCCTCGATTGAAGCTCGTGTACTTCCCCCACCGATGGTGAT
TTTCTTTCTCCATTTTTGTTATTAATTTGCTTTATTATCATGCATTTTCTTGTCAATGTT
GCTCATCACGATGTTCTTTCTGTTAACAGTTGAAGTACCATGACAGTGGTAAAGAGAAAA
TGGTAAATCCAAGGCTAGGACAGTGGAACATGATTGACAAGGTAAAACTGTAATTGACTA
TCATAATCTTTTTGCTCTCTGTAGGCACTTGAAACTAAATAAGAAATATGCTTCTATTTC
TCTTATAATGATAAATTATACTATACTTTACTGCTTTGCGTGTGGATGCGGAAGAAATAG
TTAGTGATGAAGATTTGTGTTTAATGCTTCTATTGTGGTTTAATGTGTGTTACCAAATTT
TGGCTGTGATATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCG
TATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTG
TCTACATTC
   
Sequence Name 05-K017512-022-211-E03C-8409
GenBank Accession LN488146 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K017512-022-211-E03C-8409
TAGATTTCCCGGACATGAAGCCATATCACAGCCAAAATTTGGTAACACACATTAAACCAC
AATAGAAGCATTAAACACAAATCTTCATCACTAACTATTTCTTCCGCATCCACACGCAAA
GCAGTAAAGTATAGTATAATTTATCATTATAAGAGAAATAGAAGCATATTTCTTATTTAG
TTTCAAGTGCCTACAGAGAGCAAAAAGATTATGATAGTCAATTACAGTTTTACCTTGTCA
ATCATGTTCCACTGTCCTAGCCTTGGATTTACCATTTTCTCTTTACCACTGTCATGGTAC
TTCAACTGTTAACAGAAAGAACATCGTGATGAGCAACATTGACAAGAAAATGCATGATAA
TAAAGCAAATTAATAACAAAAATGGAGAAAGAAAATCACCATCGGTGGGGGAAGTACACG
AGCTTCAATCGAGGCTAGTTGGGTAGTCACTGACATCCCAAACTCCTTGCTCA

Last Updated on 06.12.2018 16:11