Sequences from confirmation amplicon


Confirmation sequences for line 211F09 and hit At5g41140 are shown.
Line ID 211F09
Locus At5g41140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16469617
Primers used for PCR 17lm - 8474, show primer details
   
Sequence Name 27-K114022-0022-211-F09C-17lm
GenBank Accession LN498481 [GenBank]
Sequencing primer gene specific primer, 17lm: 5'-CGGAGGGATTAACAATCTCTGTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K114022-0022-211-F09C-17lm
CTATGGTGCTCGACGGACATTGTCGGTGGGAATCTCCGGTGTATGAGACGGTGAAGTTTC
TTCAGGATGTGAAAACAGGGAAGGTTAATCAGAGAATCTATCATCTAGTTATGTCAACGA
CGGTGAGGAAAATTAAACTTTCTCAGAGTTATGAAGAGATTTCTGAGATTACTTTTCTGA
TTAGTTTTGTTTTTGCAGGGATCTACAAAATCTGGTGTTGTAGGAGAGACTTCGATTGAT
TTTGCTGATTATGTAGATGCAATTAAGACTTGCAATGTTTCTCTTCCTCTCCAGAATTCA
AATTCAAAAGCAATGTTGCACGTTAGCTCTCTCTTTCTTTCTCTTTCTTTGCATTAATGT
TTGTCATGAGACATTAAGAGTGTTTGCATCCATAGCAAAGCTCACATGATTCTTGCTTCT
TTGTCTCTTTGAACTGTGATACTGAGATTTGAACTGGGTTGTTTCATTATCAAAGGTATA
TGCAATGGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCACAATTTA
   
Sequence Name 06-K114021-0022-211-F09C-8409
GenBank Accession LN488899 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K114021-0022-211-F09C-8409
ATTTCCATTGCATATACCTTTGATAATGAAACAACCCAGTTCAAATCTCAGTATCACAGT
TCAAAGAGACAAAGAAGCAAGAATCATGTGAGCTTTGCTATGGATGCAAACACTCTTAAT
GTCTCATGACAAACATTAATGCAAAGAAAGAGAAAGAAAGAGAGAGCTAACGTGCAACAT
TGCTTTTGAATTTGAATTCTGGAGAGGAAGAGAAACATTGCAAGTCTTAATTGCATCTAC
ATAATCAGCAAAATCAATCGAAGTCTCTCCTACAACACCAGATTTTGTAGATCCCTGCAA
AAACAAAACTAATCAGAAAAGTAATCTCAGAAATCTCTTCATAACTCTGAGAAAGTTTAA
TTTTCCTCACCGTCGTTGACATAACTAGATGATAGATTCTCTGATTAACCTTCCCTGTTT
TCACATCCTGAAGAAACTTCACCGTCTCATACACCGGAGATTCCCACCGACAATGTCCGT
CGAGCACCATAGCCTTCTCTGCCTTCCCCGTCGACTTCCCAACATCTCCGGGAACTACAG
AGATTGTTAATCCCTCC

Last Updated on 06.12.2018 16:11