Sequences from confirmation amplicon


Confirmation sequences for line 212C11 and hit At2g19230 are shown.
Line ID 212C11
Locus At2g19230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8345987
Primers used for PCR JD11 - 8474, show primer details
   
Sequence Name 08-K102723-0022-212-C11C-8409
GenBank Accession LN489686 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K102723-0022-212-C11C-8409
TTTACAATTGAATTATTTTTGAATGTACTTTTCCGAAGCCTCCTTGGCCAAGAACTCTCT
CAAAGTTGTTTGTAATTTCCACTATTTCTGAATACTTGTAGTATCGCTTTGTGTCTAGTG
GTCCGGTCTTGACACCTGCTAAGAGATCAATGAGAAATTATTTTCATCAAAAGTCTTGGA
AAAAATAAGCAATTTTAACAGGTGTTTTATGATTCACCCACTTTGTTGTCTCTTCTTGAA
TTGCCAGAAAGAAATTAATGCGAGCAAGAGAAAAAACAATCCTGTAACAGATGCTACTGA
TGGAATAATGTATTCTTTCCTCTCCGTCTTTTTGTTTCGACATGAATCAG
   
Sequence Name 24-K102728-0022-212-C11C-JD11
GenBank Accession LN496948 [GenBank]
Sequencing primer gene specific primer, JD11: 5'-TCTGATTCATGTCGAAACAAAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K102728-0022-212-C11C-JD11
CATCTGTTACAGGATTGTTTTTTCTCTTGCTCGCATTAATTTCTTTCTGGCAATTCAAGA
AGAGACAACAAAGTGGGTGAATCATAAAACACCTGTTAAAATTGCTTATTTTTTCCAAGA
CTTTTGATGAAAATAATTTCTCATTGATCTCTTAGCAGGTGTCAAGACCGGACCACTAGA
CACAAAGCGATACTACAAGTATTCAGAAATAGTGGAAATTACAAACAACTTTGAGAGAGT
TCTTGGCCAAGGAGGCTTCGGAAAAGTACATTCAAAAATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCAA
ATGGG
Primers used for PCR KC86 - 3144, show primer details
   
Sequence Name 05-K106971-0022-212-C11C-3144
GenBank Accession LN488375 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K106971-0022-212-C11C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTGTCT
TACGTGGGGAACAAGTTGCTATCAAGATGTTGTCTAAATCATCAGCTCAAGGCTACAAAG
AATTCCGAGCAGAGGTTACTCTCATATAGTCTTAAAGGTTCAGTCCAGATGAAAAGTGAA
CTATTCTGAGAGAGACATGGACTTTTTGATTGTCAGGTTGAACTTCTCCTGAGAGTTCAT
CACAAGAACCTAATAGCTCTTATTGGATACTGCCACGAAGGAGATCAAATGGCACTTATC
TACGAGTATATCGGTAATGGGACCTTGGGAGACTACTTGTCAGGTCAGCCTGATGTAGTC
AATTTTTG
   
Sequence Name 21-K106972-0022-212-C11C-KC86
GenBank Accession LN495681 [GenBank]
Sequencing primer gene specific primer, KC86: 5'-CAAAAATTGACTACATGAGGCTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K106972-0022-212-C11C-KC86
ATTACCGATTACTCGTAGATAGTGCCATTTGATCTCCTTCGTGGCAGTATCCAATAAGAG
CTATTAGGTTCTTGTGATGAACTCTCAGGAGAAGTTCAACCTGACAATCAAAAAGTCCAT
GTCTCTCTCAGAATAGTTCACTTTTCATCTGGACTGAACCTTTAAGACTATATGAGAGTA
ACCTCTGCTCGGAATTCTTTGTAGCCTTGAGCTGATGATTTAGACAACATCTTGATAGCA
ACTTGTTCCCCACGTAAGACAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCT
GATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTC
TTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCA

Last Updated on 06.12.2018 16:11