Sequences from confirmation amplicon


Confirmation sequences for line 212D11 and hit At3g06455 are shown.
Line ID 212D11
Locus At3g06455 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1981765
Primers used for PCR 06vi - 8474, show primer details
   
Sequence Name 18-K117806-0022-212-D11C-06vi
GenBank Accession LN494494 [GenBank]
Sequencing primer gene specific primer, 06vi: 5'-AAGTTTAGGCTTGGTGACTGAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K117806-0022-212-D11C-06vi
TTTTAAACATTTGGGCTTAGAACCCATTTCGGTAGGGACTTAACCAAAGATAACTGAAAC
ATTTGAACTCCTAGGCCTTGTCATCTAACACACATTTCTTATTTACATAATGGCTTGTAT
GATTTGAACTACAAGCCAAAAAACTTCAAAAGGGTGTAATCTTTTTGAAGATGGATGTTG
GTCTTCATTTCTTGGCCAGTAATTTCTTCGGGAGCTTATCGAGGGGCGTTGATTTAAGTA
AAAATAGCCTCGCAGCTCGCTCTCGCAATGTCCCTCTGCATTTCAGACCGCGTGACTGAA
GCTCAGTCTTCAATCTCTCCATCCCCAAAACCTCCATATCTGTGGCTGCTTTGAACTCAG
CAAAGTTAAGCGGTTCAACAGGTTTGCAACACACTCTTTCTTTCGCAGTTCCTCCTCTCT
TTACGACAAAATCATCGTTTCCACTTGATTTCTCCACCTTTTCAAGTTGATCCATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 18-K117784-0022-212-D11C-8409
GenBank Accession LN494493 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K117784-0022-212-D11C-8409
CATTTCATGGATCAACTTGAAAAGGTGGAGAAATCAGTGGAAACGATGATTTTGTCGTAA
AGAGAGGAGGAACTGCGAAAGAAAGAGTGTGTTGCAAACCTGTTGAACCGCTTAACTTTG
CTGAGTTCAAAGCAGCCACAGATATGGAGGTTTTGGGGATGGAGAGATTGAAGACTGAGC
TTCAGTCACGCGGTCTGAAATGCAGAGGGACATTGCGAGAGCGAGCTGCGAGGCTATTTT
TACTTAAATCAACGCCCCTCGATAAGCTCCCGAAGAAATTACTGGCCAAGAAATGAAGAC
CAACATCCATCTTCAAAAAGATTACACCCTTTTGAAGTTTTTTGGCTTGTAGTTCAAATC
ATACAAGCCATTATGTAAATAAGAAATGTGTGTTAGATGACAAGGCCTAGGAGTTCAAAT
GTTTCAGTTATCTTTGGTTAAGTCCCTACCGAAATGGGTTCTAAGCCCAAATGTTTTAAA
AACCTATAGCCTTATTTGAAAAAACGACAATTTTCAGTCACCAAGCCTAAACT

Last Updated on 06.12.2018 16:11